RAPID DETECTION OF TOXIC ALGAE USING UV RESONANCE RAMAN

目标是鉴定各种荧光生化和 用荧光法测定膀胱组织中的形态成分 显微镜。对膀胱组织的冰冻切片进行成像 荧光显微镜。连续切片被染色并由 病理学家。在370 nm和400 nm激发下，浸润性癌 很少的荧光，除了看起来很小的散布的 胶原蛋白碎片和偶尔的炎性细胞。对于另一种 组织诊断，上皮细胞要么没有，要么检测不到 荧光。粘膜下层的胶原丝，呈蓝色 弦是主要的荧光团，尽管偶尔会 黄绿色的斑点似乎也是炎性细胞 出现了。浸润性癌缺乏荧光解释了 观察到的大大减弱的荧光光谱。不存在 根据观察结果，预计不会出现上皮荧光 早期结肠内发育不良的隐窝细胞荧光。在图像中 Ta Ken的CIS，粘膜下层显示较少的荧光胶原 链，但鉴于最近的膀胱切除术结果，相应的 染色的玻片需要仔细检查是否有水肿或出血。 这可能在粘膜下层的这种变化中发挥了作用。分析和 对这些显微镜图像的解释仍在进行中。

The objective is identify various fluorescent biochemical and morphological components in bladder tissues using fluorescence microscopy. Frozen sections of bladder tissue were imaged with a fluorescent microscope. Serial sections were stained and reviewed by a pathologist. At 370 nm and 400 nm excitation, invasive cancer had little fluorescence other than what appeared to be scattered tiny collagen fragments and occasional inflammatory cells. For the other tissue diagnoses, epithelium had either no or undetectable fluorescence. Collagen strands in the submucosa, appearing as blue strings, were the primary fluorophore, although occasional yellowish-green spots that seem to be inflammatory cells also appeared. The lack of fluorescence of the invasive cancer explains the much weakened fluorescence spectra observed. The absence of epithelial fluorescence was not expected based on the observation earlier of dysplastic crypt cell fluorescence in the colon. In images ta ken of CIS, the submucosa exhibited fewer fluorescencing collagen strands, but in view of recent cystectomy results, the corresponding stained slides need to be closely checked for edema or hemorrhage which might play a role in this change in the submucosa. Analysis and interpretation of these microscope images is still ongoing.