REVERSE PHARMACOLOGY

大卫·德鲁宾和细胞极性联盟的其他成员 提出了构建激活结构域和结合结构域 约100种蛋白质的融合体参与芽的发育和细胞 师. 所有可能的配对都将进行系统测试， 双杂交相互作用 该财团的每个成员都已 分配了一些诱饵来建造。 当诱饵被 构建，我们将使激活结构域融合构建体或 从酵母激活结构域阵列中挑选它们， 分析PCR和测序。 以下基因已经被 本实验拟包括的蛋白质： 信号传导：CDC 42，CDC 42-GTP，CDC 42-GDP，CDC 24，BOI 1，BOI 2，RH 0 1， RHO 2、RHO 3、RHO 4、ROM 1、MSB 3、MSB 4、BEM 1、STE20、CLA4、SKM 1、GIC 1、 GIC 2、BEM2、BEM3、RGA 1、MSB 1、MSB 2、BNI 1、RSR1、BUD 2、BUD 5、ZDS 1、 ZDS 2，FAR 1。 肌动蛋白和相关蛋白：ABP 1、CAP 1、CAP 2、SRV 2， SLA 2、SLA 1、COF 1、CRN 1、MYO 1、MYO 2、MYO 3、MYO 4、MYO 5、SAC 6、ACT 1， ARP 2、ARP 3、RVS167、AIP 1、AIP 2、AIP 3/BUD 6、PFY 1、LAS 17、IQG 1。 参与分泌的蛋白质：SEC 1、SEC 2、SEC 3、SEC 4、SEC 5、SEC 6， SEC 8、SEC 9、SEC 10、SEC 15、SNC1、SNC2、SSO 1、SSO 2。 隔膜：CDC 3， CDC 10，CDC 11，CDC 12，SEP 7，BUD 3，BUD 4芽位相关蛋白 选择：BUD 3，BUD 4，BUD 7，BUD 8，BUD 9，BUD 10我们到目前为止 接受下列蛋白质的Gal 4-DNA结合结构域构建体 来自Clarence Chan：GIC 1，GIC 2，MSB 2，BEM 3，RGA 1和RSR 1。

David Drubin and other members of the Cell Polarity consortium have proposed constructing activation domain and binding domain fusions of about 100 proteins involved in bud development and cell division. All possible pairs will be tested systematically for two-hybrid interactions. Each member of the consortium has been assigned a number of baits to construct. While baits are being constructed, we will make the activation domain fusion constructs or pick them from the yeast activation domain array and verify them by analytical PCR and sequencing. The following genes have been proposedto be included in this experiment: Proteins involved in signalling: CDC42, CDC42-GTP, CDC42-GDP, CDC24, BOI1, BOI2, RHO1, RHO2, RHO3, RHO4, ROM1, MSB3, MSB4, BEM1, STE20, CLA4, SKM1, GIC1, GIC2, BEM2, BEM3, RGA1, MSB1, MSB2, BNI1, RSR1, BUD2, BUD5, ZDS1, ZDS2, FAR1. Actin and associated proteins: ABP1, CAP1, CAP2, SRV2, SLA2, SLA1, COF1, CRN1, MYO1, MYO2, MYO3, MYO4, MYO5, SAC6, ACT1, ARP2, ARP3, RVS167, AIP1, AIP2, AIP3/BUD6, PFY1, LAS17, IQG1. Proteins involved in secretion: SEC1, SEC2, SEC3, SEC4, SEC5, SEC6, SEC8, SEC9, SEC10, SEC15, SNC1, SNC2, SSO1, SSO2. Septins: CDC3, CDC10, CDC11, CDC12, SEP7, BUD3, BUD4 Proteins involved in bud site selection: BUD3, BUD4, BUD7, BUD8, BUD9, BUD10 We have so far received Gal4-DNA binding domain constructs of the following proteins from Clarence Chan: GIC1, GIC2, MSB2, BEM3, RGA1, and RSR1.