NACL/FLUID SECRETION BY MAMMALIAN RENAL COLLECTING DUCTS

细胞外液量的维持需要平衡NaCl摄入及其从体内的排泄。 已经确定哺乳动物肾脏中的肾小管和集合管在尿液形成期间重吸收大量NaCl和液体。 然而，一些研究表明，许多肾小管节段也可能具有用于净溶质和液体分泌的分子组分。 因此，盐和液体平衡可能是大规模吸收和分泌微调的结果。 内髓集合管（IMCD）的理想位置是在尿液离开肾脏之前对尿液进行最后的调整。 来自IMCD的细胞具有顶端囊性纤维化跨膜传导调节因子（CFTR）Cl通道，并且来自大鼠IMCD细胞的原代培养物显示由cAMP刺激的产电阴离子分泌。 据我所知，氯化钠和液体分泌的人肾单位还没有得到充分的检查。 我将研究人类肾小管，特别是IMCD，可以积极分泌（Na）Cl和液体，从而有助于ECV的调节的程度。

The maintenance of the extracellular fluid volume requires a balance NaCl intake and its excretion from the body. It is well established that renal tubules and collecting ducts in the mammalian kidney reabsorb large amounts of NaCl and fluid during urine formation. However, several studies suggest that many tubular segments may also have the molecular components for net solute and fluid secretion. Therefore, it is possible that salt and fluid balance is a consequence of large scale absorption and fine-tuning by secretion. The inner medullary collecting duct (IMCD) is ideally positioned to make the final adjustments to urine before it leaves the kidney. Cells from the IMCD possess apical cystic fibrosis transmembrane conductance regulator (CFTR) Cl channels and primary cultures from rat IMCD cells display electrogenic anion secretion stimulated by cAMP. To my knowledge, NaCl and fluid secretion by the human nephron have not been adequately examined. I will investigate the extent to which human renal tubules, notably the IMCD, can actively secrete (Na)Cl and fluid and thereby contribute to the regulation of ECV.