OOCYTE CHROMATIN ORGANIZATION IN FOLLICULOGENESIS & OOGENESIS IN M MULATTA

从氟烷下摘除的卵巢中收集卵泡 麻醉7只恒河猴，观察卵泡和卵子直径。 用荧光法测定和测定染色质组织 用显微镜观察卵母细胞和卵泡的相对生长曲线 黄土回归技术表现为三相曲线。 卵泡与卵母细胞生长和成熟的关系 (核周染色质构型)分析染色质 在卵泡方面，组织与卵子大小有关 大小，卵母细胞首先作出反应(超过无边框核仁期) 最早的鼻腔形成时期，然后反应保持 相对相同，直到卵泡长到600米时多数 的应答者处于部分边缘阶段，但直到 卵泡直径超过1000米的大多数 卵母细胞处于完全有边框的阶段(有减数分裂能力)

Follicles were collected from ovaries removed under halothane anaesthesia from 7 rhesus monkeys Follicle and ovum diameters were measured and chromatin organization was determined by fluorescence microscopy The relative growth curve of oocytes and follicles using a loess regression technique displayed a triphasic curve The relationship of follicle and oocyte growth and oocyte maturation (perinucleolar chromatin configuration) was analyzed Chromatin organization progressed in relation to ovum size In terms of follicle size, oocytes first respond (past the unrimmed nucleolar stage) during the period of earliest antrum formation, then the response remains relatively the same until the follicle reaches 600 m when the majority of responders are at the partial rim stage But it is not until the follicle has reached a diameter beyond 1000 m that the majority of oocytes are at the fully rimmed stage (meiotically competent)