STRUCTURE OF SUP35 FIBERS: YEAST PRION INHERITANCE

已知腺病毒蛋白酶结合双链DNA， 它被认为是把一个弯曲到它。蛋白质是小的（只有 211个氨基酸），因此不太可能在STEM中看起来很像。 中 初步实验，一个300 bp的DNA片段与蛋白质结合 检查DNA是否“扭结”。 不知何故， 所以实验会重复进行。 的 蛋白酶活性被DNA和蛋白质辅因子修饰。 一 蛋白酶上的半胱氨酸是Au标记的。 我们希望通过观察 在STEM中结合不同的辅因子来定位 复合物中的蛋白酶

An adenovirus protease is known to bind to double stranded DNA, and it is believed to put a bend into it. The protein is small (only 211 amino acids) so is unlikely to look like much in the STEM. In a preliminary experiment, a 300 bp piece of DNA with the protein bound was examined to see if the DNA was "kinked". Somehow, there was too little DNA present, so the experiment will be repeated. The proteinase activity is modified by DNA and protein cofactors. A cysteine on the protemase was Au-labeled. The hope is to look at it in the STEM in conjunction with different cofactors to locate the proteinase in the complex.