FOLDING OF YEAST CYTOCHROME C

为了了解两者之间的相互作用机制 胰凝乳酶及其抑制剂OMTKY3，有必要与 OMTKY3突变体对其结构的生化行为 属性。用核磁共振进行pH滴定可以提供这样的信息。通过 通过pH滴定的方法，可以得到不同氨基的pKa值 酸残留物。通过比较这些pKa值，野生型的pKa值 和其他突变体的基因，我们可以获得重要的结构 为OMTKY3提供信息，并进一步了解其 对凝乳酶的抑制作用。

In order to understand the mechanism of the interaction between chymotrypsin and its inhibitor - OMTKY3, it is necessary to relate the biochemical behavior of the mutants of OMTKY3 to their structural properties. pH titration by NMR can provide such information. By means of pH titration, we can obtain the pKa value of different amino acid residues. By comparison of these pKa values,, those of wild type and those of other mutants, we can obtain important structural information for OMTKY3 and arrive at a further understanding of its inhibitory action on chymotrypsin.