MAGNETIC ALIGNMENT OF CLOSTRIDIUM PASTEURIANUM RUBREDOXIN

碳水化合物的标准一维质子核磁共振波谱 反常质子和其他几个位置的可识别信号 特例。剩余的糖质子共振被分组 一起形成一个未解的信封。COSY光谱可以用来 识别并分配这些包络中的个体共振 发出星团信号。氨基酸残基间的连接和全序列 碳水化合物可以用NOESY光谱分析。这些化合物要 被分析的是环多半乳糖醛酸环的混合物 大小为5，6，7和8。这些化合物类似于？-，？-， -环糊精，相对分子质量900-1400Da。可能的 结构可以是-1，2或-1，4糖苷键。舒适的光谱将会 用于指定每个糖环的质子共振，以及 对称的非对角线NOE串音可能有助于确定 残基间连锁。如果h1和h2或h1之间存在串音 和H4连接在两个连接的糖环上，则糖链可以 被明确分配。

Standard 1D proton NMR spectrum of carbohydrates exhibit recognizable signals for anomeric protons and a few other sites in special cases. The remaining sugar proton resonances are grouped together to form an unresolved envelope. COSY spectra can be used to recognize and assign the individual resonances in these enveloping signal clusters. The interresidue linkage and overall sequence of carbohydrates can be analyzed by a NOESY spectrum. The compounds to be analyzed are a mixture of cyclopolygalacturonic acids with ring sizes of 5,6,7, and 8. These compounds are similar to ?-, ?-, ?-cyclodextrins with a molecular weight of 900-1400 Da. The possible structure may be a-1,2 or a-1,4 glycosidic linkage. COSY spectra will be used to assign the proton resonance for each sugar ring, and symmetric off-diagonal NOE crosspeaks may help to determine the interresidue linkage. If there is a crosspeak between H1 and H2 or H1 and H4 on two connecting sugar rings, then the glycosydic linkage can be clearly assigned.