MOLECULAR MARKERS FOR SQUAMOUS CELL CARCINOMA

摘要鳞状细胞癌是口腔最常见的恶性肿瘤。 空洞。未能诊断和治疗早期损害，尽管他们很好 明确的组织病理学标准和临床检测的可及性； 突出的是先进的表现和显著的发病率 这种癌症患者的死亡率。自发展以来， 肿瘤的进展是由于各种不同的 基因改变，识别与早期， 中晚期口腔鳞状病变将有重要的 诊断和临床意义。P53基因和，基于我们的 初步数据，精选染色体基因座，重点是精制 利用微卫星标记定位3p21、8p21、9p21和11p15.5区域 将在正常和发育异常的显微解剖样本上进行分析 每个标本的上皮组织和侵袭性病变。在第一阶段 本研究，100例回顾性病例的样本将形成我们的材料 为每一项确定最高和最一致的标记 病理形态分期。第二阶段，每期20个/年 预期切除的标本将仔细和系统地进行 映射为正常、不同Pre的多个空间分离样本 恶性上皮细胞与恶性病变的克隆性判定 所选标记的进展性、稳定性和异质性。这个 结果将与组织病理进展、侵袭性相关 肿瘤特征和流行病学因素。

Squamous cell carcinoma (SCC) is the most common malignancy of the oral cavity. Failure to diagnose and treat early lesions, despite their well defined histopathologic criteria and accessibility to clinical detection, underlies the advanced presentation and the significant morbidity and mortality of patients with this cancer. Since the development and progression of neoplasms result from continuous accumulation of various genetic alterations, identifying genetic markers associated with early, intermediate and advanced oral squamous lesions will have important diagnostic and clinical implications. p53 gene and, based on our preliminary data, selected chromosomal loci with emphasis on the refined mapping of 3p21, 8p21, 9p21 and 11p15.5 regions by microsatellite markers will be analyzed on microdissected samples of normal and dysplastic epithelium and invasive lesions from each specimen. In the first phase of the study, samples from 100 retrospective cases will form our materials to determine the highest and most consistent markers for each pathomorphologic stage. In the second phase, each of 20/year prospectively resected specimens will be carefully and systematically mapped for multiple spatially separate samples of normal, different pre- malignant epithelium and malignant lesions to determine clonal progression, stability and heterogeneity of the selected markers. The results will be correlated with histopathologic progression, aggressive tumor characteristics and epidemiological factors.