FIELD-APPLICABLE SEROLOGIC DIAGNOSIS AND EXPERIMENTAL CONTROL STRATEGIES

人类猪带绦虫囊虫病是一种严重虚弱的疾病，但 可治愈的疾病。在猪身上，这种感染占了数百万 每年造成的经济损失高达10美元。初步流行病学调查 随着新开发的免疫印迹试验(EITB)在许多拉丁美洲 国家、中国和印度表示，这是一个意义重大的公众 健康问题，人类患病率从8%到15%不等， 在猪中高达45%。 美国疾病控制中心最近的一项调查显示，内源性传播 在有移民家庭佣工的家庭中，导致血清- 仅在布鲁克林一个社区的患病率就高达13%。囊虫病 因此，获得了一种国内模式。对这一重要公众的控制 健康问题取决于患者的识别和治疗 还有猪。 我们建议扩展和增强功能、有用性和实用性 我们独特的化验/现场操作系统用于检测和 控制这一重要疾病。我们还建议测试其疗效 一种新的猪/人方法来监测和控制 囊虫病。 我们将：1.开发低成本、健壮、适用于现场的版本 人和猪囊虫病2的EITB检测方法， 确定接受治疗的患者感染特异性抗体的寿命 患者通过定量同型分析3，以检验假设一个 一次处理EITB阳性猪，同时取消胶带 在血清阳性的人类患者中，蠕虫和排泄是一种可行和 控制人和猪囊虫病的有效途径4， 确定无囊虫病猪的血清学是否有用和 衡量疾病流行和控制的有效哨兵 人类囊虫病的有效性。

Human Taenia solium cysticercosis is a severely debilitating, yet treatable disease. In the pig, this infection accounts for multimillion dollars of economic loss per year. Preliminary epidemiological surveys with the newly developed immmunoblot assay (EITB) in many Latin American countries, China, and India indicated that this is a significant public health problem with prevalence rates ranging from 8% to 15% in humans and up to 45% in pigs. A recent Centers for Disease Control Survey showed endogenous transmission in families with immigrant domestic workers, resulting in a sero- prevalence of 13% in a single community in Brooklyn alone. Cysticercosis has therefore, acquired a domestic mode. Control of this important public health problem is dependent on the identification and treatment of patient and pigs. We propose to extend and enhance the power, usefulness, and practicality of our unique assay/field operation system toward the detection and control of this important disease. We also propose to test the efficacy of a novel pig/human approach to surveillance and control of cysticercosis. We will: 1. To develop a low cost, robust, and field applicable version of the EITB assay for the detection of human and pig cysticercosis 2, determine the longevity of infection-specific antibodies in treated patients by quantitative isotype assays 3, To test the hypothesis that a single treatment of EITB positive pigs, together with elimination of tape worms in sero-positive human patients, and eduction is a viable and effective approach to the control of human and pig cysticercosis 4, to determine if the serology of cysticercosis-free pigs is a useful and effective sentinel for measuring disease prevalence and control effectiveness of human cysticercosis.