CORE--MICROSCOPY AND IMAGING FACILITY

显微镜和成像提供仪器、培训和 光学和电子显微镜图像分析方面的专家建议。 光学显微镜包括明场光学、暗场光学、 相位对比度、差分干涉对比度或霍夫曼 光学、荧光广域和共焦显微镜 肿瘤细胞、组织和组织的分辨率光学显微镜 亚细胞定位。透射式和扫描式电子 显微镜可用于高分辨率的亚细胞形态。 一种新获得的微注射系统使我们能够微注射 单个肿瘤细胞。该系统上的倒置显微镜是 与摄像机接口，用于数字化图像。安装 兼容Windows的Optimas 5.1更新了我们的映像容量 分析。运动细胞的时间推移成像现在正在进行中，使用 这个工作台。共享设施已经大大扩展了 最近收购的容量或图像存储和传输 可写式光盘与数字化影像工作的网络化 站台。除了核心支持的传统研究外， 图像分析/微量注射最新项目的一些例子 包括显微注射质粒和反义基因系统 寡核苷酸，核大小和形状的量化 体外血管生成，活细胞运动，大小和程度 小鼠乳腺发育过程中的上皮分枝和细胞 表面结构。苏塞特·米勒博士是该中心的董事兼经理 共享资源，戴茂正博士是显微镜专家。 共享源代码的主实验室现在位于 在癌症中心的四楼空间内的新研究 大楼。电子显微镜和共焦显微镜各有特点。 原址在毗邻的临床前科学大楼。这个 在过去的三年中，该设施的使用范围显著扩大 几年来，首席调查员的人数大约翻了一番(15人 至33个)、同行评审项目(15至34个)和部门(8至 13)利用共享资源。资源对所有8个资源都起作用 程序和其他4个共享资源。

Microscopy and Imaging provides instrumentation, training, and expert advice for light and electron microscopy d image analysis. Light microscopy includes optics for bright field, dark field, phase contrast, differential interference contrast or Hoffman optics, epifluorescence wide field and confocal microscopy for high resolution light microscopy of tumor cells, tissues, and subcellular localizations. Transmission and scanning electron microscopes available for high resolution subcellular morphology. A newly acquired microinjection system allows us to microinject individual tumor cells. The inverted microscope on this system is interfaced with a camera for digitizing images. The installation of Windows-compatible Optimas 5.1 updates our capacity for image analysis. Time lapse imaging of motile cells is now underway using this work station. The shared facility has a greatly expanded capacity or image storage and transfer with the recent acquisition of writable CD-ROM and networking of the digitized imaging work stations. In addition to traditional studies supported by the core, some examples of recent projects the image analysis/ microinjection system include microinjection of plasmids and antisense oligonucleotides, quantification of nuclear size and shape, in vitro angiogenesis, live cell motility, size and extent of epithelial branching in developing mouse mammary glands, and cell surface structure. Dr. Susette Mueller is the Director and Manager of the Shared Resource, and Dr. Maozheng Dai is the Microscopist. The main laboratory of the shared source is now located on the fourth floor in the Cancer Center space within the new Research Building. The electron and confocal microscopes are m their original location in the adjacent Preclinical Science Building. The use of the facility has expanded significantly in the past three years, with about double the number of principle investigators (15 to 33), peer-reviewed projects (15 to 34), and departments (8 to 13) utilizing the Shared Resource. The Resource in-acts with all 8 programs and with 4 other Shared Resources.