Towards successfully realising the impact of the chip-based phospholipid on mercury (Hg) device as a toxicity sensing system

关键词：膜基传感器；膜的渗透性和损伤；交叉验证/演示；技术开发；人员交流；项目工作本项目可分为以下四类活动，最终目的是将有趣和独特的技术转让给适当的最终用户；SEAC、联合利华和ALCONTROL有限公司及其同事；IBMT、Fraunhofer：-(I)交叉验证/演示。利兹大学开发了一种独特而优雅的生物传感器，它对破坏生物膜和/或渗透到生物膜上的化合物和颗粒敏感，这一特性被定义为生物膜活性。因此，这项任务的目的是准确评估利兹生物传感器试验与最终用户目前在实现相同或相似功能方面所采用的方法进行比较。这项活动将明确界定利兹公司和最终用户的技术各自的优点和缺点。这是必要的，因为虽然利兹生物传感器还处于原型阶段，但在检测一组常见化合物的生物膜活性方面，将其性能与其他系统进行比较是至关重要的。将特别注意每种技术测量的参数的相似性和差异性。这项活动的结果是：(A)使最终用户能够确定将这项技术添加到他们的测量系统中是否有利，以及(B)使利兹大学能够使他们的技术适应特定的应用。其影响是用户社区对利兹设备的能力越来越有信心和接受。技术发展。这项技术开发将把利兹生物传感器从原型阶段转变为可由熟练技术人员操作的常规传感装置。这一改进将包括简化数据分析，并将设备从一个模块扩展到三个或更多模块，这将使其能够用于高吞吐量的自动化配置。因此，这项活动的结果是将利兹生物传感器系统从一个实验室原型转变为一个可由最终用户实验室中的熟练技术人员以他们指定的形式使用的系统。其影响是一种日益强大的生物传感设备，其应用中的感知风险已降低，从而使最终用户更容易接受。交换和安置。这一目标将使(A)终端用户科学家能够操作并完全熟悉利兹生物传感器，以及(B)来自利兹的科学家能够全面评估终端用户的方法和要求，以便准确地评估终端用户技术如何与利兹生物传感器技术相适应。这项活动的结果是将利兹技术转让给最终用户，作为他们自己的免费系统。目前，利兹生物传感器尚未被最终用户采用。这一目标将使利兹生物传感器具有直接的用户应用，这不仅扩大了用户的设施，而且验证和巩固了利兹生物传感器的适用性。其影响是将技术转让给最终用户。促进了传播。这一目标将使利兹的科学家能够向最终用户传达项目进展情况，并让最终用户对交付成果发表意见。项目结束后将举行研讨会，介绍项目的最终报告。与外部参与者一起参加的研讨会的开放性将确保其他潜在最终用户能够越来越有信心地评估Nelson的技术，并判断它是否适合他们的需求，从而扩大其范围。这项活动的结果和影响是促进利兹技术的进一步知识转让以及除SEAC和ALCONTS之外的其他商业用户采用该技术。

Keywords: membrane-based sensor; membrane permeability and damage; cross-validation/demonstration; technical development; staff exchange; project workshopThis project can be divided into the following four categories of activity with the ultimate aim of transferring an interesting and unique technology to appropriate end users;SEAC, Unilever and ALcontrol Ltd and co-workers; IBMT, Fraunhofer:- (i) Cross-validation/demonstration. The University of Leeds has developed a unique and elegant biosensor which is sensitive to compounds and particles which damage and/or are permeable in biological membranes a property defined as biomembrane activity. The aim of this task therefore is to evaluate exactly how the Leeds biosensor assay compares with those currently employed by the end users in achieving the same or similar functions. This activity will provide a clear definition of the respective advantages and disadvantages of both the Leeds and the end users' techniques. This is necessary since although the Leeds biosensor is at prototype stage, it is essential that its performance is compared with other systems in their detection of the biomembrane activity of a common group of compounds. Particular attention will be paid to the similarity and differences in the parameters being measured by each technique. The outcome of this activity is, (a) to enable end users to determine whether it is advantageous to add this technology to their measuring systems and, (b) to enable Leeds to adapt their technology to specified applications. The impact is an increasing confidence in and acceptance by the user community of the capabilities of the Leeds device.2. Technical development. The technical development will take the Leeds biosensor from the prototype stage to a routine sensing device which can be operated by skilled technicians. This improvement will include streamlining the data analysis and extending the device from one module to three or more modules which will will enable it be used in a high-throughput automated configuration. The outcome of this activity therefore is to transform the Leeds biosensor system from a lab prototype into one which can be used by skilled technicians in the end user laboratories in a form which is specified by them. The impact is an increasingly robust biosensing device where a perceived risk in its application has been decreased allowing a more ready take up by end users.3. Exchanges and placements. This objective will allow (a) end user scientists to operate and become completely familiar with, the Leeds biosensor and (b) scientists from Leeds to fully evaluate the end-users' methods and requirements in order to assess exactly how the end user technology fits in with the Leeds biosensor technology. The outcome of this activity is to transfer the Leeds technology to end users as a complimentary system to their own. Currently the Leeds biosensor has not been adopted by an end user. This objective will enable the Leeds biosensor to have a direct user application which not only expands the user's facility but also validates and consolidates the Leeds biosensor's applicability. The impact is a transfer of the technology to the end user.4. Facilitated dissemination. This objective will enable the Leeds scientists to communicate the project progress to the end users and for the end users to comment on the deliverables. An end of project workshop will be held where the project's final report will be presented. The open nature of the workshop with outside participants will ensure other potential end users can assess Nelson's technology with increasing confidence and judge whether it is suitable for their needs so expanding its remit. The outcome and impact of this activity is to facilitate further knowledge transfer of the Leeds technology and its take up by other commercial users in additional to SEAC and ALcontrol.