BIOLOGICAL MASS SPECTROMETRY SYSTEMS

最先进的基质辅助激光解吸飞行时间 （MALDI-TOF）和台式电喷雾电离（ESI）质量 将购买光谱仪，以进一步研究 设施，博士后助理和研究生在 化学系、生物化学系、细胞与结构系 生物学 该系统将用于生物学研究 高分子量的大分子。 存在一系列 用现有质量无法进行的重要测量 光谱仪，因此，这一购买成为使广泛的 的项目。 新仪器将被安置在学校的 化学科学在其质谱实验室。 该系统的主要用户将是M。吉列学习角色 肽在大脑中的生物钟，J.A.卡策内伦博根 分析配体诱导的构象变化和磷酸化 雌激素受体D. Kranz研究T细胞的控制 与肽/蛋白质激动剂和拮抗剂的活性，J.S.摩尔到 表征结构控制的大分子，K.L.莱因哈特， 高级高分辨率生物质谱苏斯利克至 研究血红素蛋白和金属卟啉，和J. V. Sweedler， 研究肽在整个细胞中的分布， 单个神经元的过程。 Sligar教授，虽然只是一个未成年人 拟议文书的用户，是学校的主任， 伊利诺伊大学的化学科学，因此是 本提案的主要研究者。

State-of-the-art matrix-assisted laser desorption time-of-flight (MALDI-TOF) and bench-top electrospray ionization (ESI) mass spectrometers will be purchased to further the research of the facility, postdoctoral associates, and graduate students in the Departments of Chemistry, Biochemistry, and Cell and Structural Biology. The systems will be used for the study of biological macromolecules of high molecular weight. There are a series of important measurements that cannot be undertaken with current mass spectrometers, and so this purchase becomes enabling for a wide range of projects. The new instruments will be housed in the School of Chemical Sciences in its Mass Spectrometry laboratory. The major users of the systems will be M. Gillette to study the roles of peptides in the brain~s biological clocks, J.A. Katzenellenbogen to analyze ligand-induced conformational changes and phosphorylation in estrogen receptors, D. Kranz to investigate the control of T-cell activity with peptide/protein agonists and antagonists, J.S. Moore to characterize structure-controlled macromolecules, K.L. Rinehart to advance high resolution biological mass spectrometry, K.S. Suslick to study heme proteins and metalloporphyins, and J.V. Sweedler to investigate the distribution of peptides throughout the cellular processes of individual neurons. Prof. Sligar, although only a minor user of proposed instruments, is the director of the School of Chemical Sciences at the University of Illinois and therefore is the Principal Investigator of this proposal.