DIGITAL CONFOCAL FACILITY AND SATELLITES

八个主要用户申请资金建立中央数字 反褶积成像站和两个卫星设施。这个 主要需求是获取定量准确的共焦图像， 但要特别避免激光照射的有害影响 光漂白和在活组织中的光毒性。主站 将包括一个表观荧光显微镜，两个可互换的 冷却的ccd摄像机、计算机控制的工作台(X、Y和Z轴)、 活细胞舞台孵化器，Silicon Graphics R8000计算机 用于图像去卷积，以及彩色打印机。 此外，我们还将在健康中心建立两个卫星设施 科学中心。它们将能够进行常规的图像采集， 并将通过以太网连接到主站，从而 图像可以在中心站去卷积。卫星将会 适用于不需要特殊能力的工作 中央车站。每个都将包括一个显微镜，Z轴步进器 马达、摄像头、计算机和以太网链路。通过这种方式，我们将成为 能够最大限度地利用这项技术。 所有八个主要用户都获得了NIH的工作资金，并且所有 拟议的项目源于目前获得资助的项目。关于 一半的主要用户拥有广泛的数字体验 图像处理和/或激光扫描共聚焦显微镜。不是 类似的仪器目前在Health Science上有售 中心。我们有八年的合议制实施历史 和以前共享仪器的管理提供了一个背景 这对当前提议的成功来说是个好兆头。

Eight Major Users request funds to establish a central digital deconvolution imaging station and two satellite facilities. The primary need is to acquire quantitatively accurate confocal images, but avoid the deleterious effects of laser illumination particularly photobleaching and in living tissues phototoxicity. The main station will comprise an epi-fluorescence microscope, two interchangeable cooled CCD cameras, a computer-controlled stage (X,Y and Z axes), a stage incubator for living cells, a Silicon Graphics R8000 computer for image deconvolution, and a color printer. In addition, we will establish two satellite facilities at the Health Sciences Center. These will be capable of routine image acquisition, and will be connected to the main station via ethernet, so that images can be deconvolved at the central station. The satellites will be useful for work that does not require the special capabilities of the central station. Each will comprise a microscope, Z-axis stepper motor, camera, computer, and ethernet link. In this way, we will be able to maximize the use of this technology. All eight Major Users receive NIH funding for their work, and all of the proposed projects arise from currently funded projects. About half of the Major Users have had extensive experience with digital image processing and/or laser scanning confocal microscopy. No comparable instrument is currently available at the Health Sciences Center. We have an eight year history of collegial implementation and management of previous Shared Instrumentation Grants a background that augers well for the success of the current proposal.