CORE--CELL CULTURE

了解囊性纤维化病理生物学的基础研究 需要持续可靠的细胞来源， 患者和未患病者。因此， CORE气道细胞培养设施是获取气道组织和培养 气道表面上皮细胞和气管支气管腺细胞。组织 用于获得正常和CF气道组织的采购系统已经 确立了习 一旦获得，将组织酶促消化， 在原代培养物中建立分散的细胞。 气管 腺细胞通过单次传代扩增。 表面上皮和 腺细胞培养物的分化特性用 光学显微镜、电子显微镜和短路测量 电流和跨上皮电阻。汇合的细胞片层是 分发给调查人员。此外，已建立的细胞系 由CORE维护和分发研究者日常使用的 细胞培养设施。细胞培养核心也将继续其 努力改善文化，特别是那些来自 气管支气管腺细胞培养基添加剂的影响（生长 因子、激素、其他化学物质）对气道腺细胞生长的影响， 将进行差异化测试。空气界面馈电和 各种生长支持物（例如，不同的过滤器、各种胶原蛋白 和细胞外基质成分）也将被评估。 这些 实验应该可以让我们确定一组相对简单的 允许完全表达离子转运和粘液的培养条件 低平板密度的分泌功能。

Basic research toward understanding the pathobiology of cystic fibrosis requires a continuing and dependable source of cells, obtained from patients with and without the disease. Thus, the primary objective of the CORE Airway Cell Culture Facility is to acquire airway tissues and culture airway surface epithelial and tracheobronchial gland cells. A tissue procurement system for obtaining normal and CF airway tissue has already been established. Once obtained, tissue is enzymatically digested and the dispersed cells are established in primary culture. Tracheobronchial gland cells are expanded through a single passage. Surface epithelial and gland cell cultures are evaluated for differentiated properties using light microscopy, electron microscopy and measurements of short circuit current and transepithelial resistance. Confluent cell sheets are distributed to investigators. Additionally, established cell lines routinely used by investigators are maintained and distributed by the CORE Cell Culture Facility. The Cell Culture CORE will also continue its efforts to improve cultures, particularly those derived from tracheobronchial gland cells. The effects of media additives (growth factors, hormones, other chemicals) on airway gland cell growth and differentiation will be tested. The effects of air interface feeding and of a variety of growth supports (e.g. different filters, various collagens and extracellular matrix components) will also be assessed. These experiments should allow us to determine a relatively simple set of culture conditions which allows full expression of ion transport and mucus secretory function from low plating densities.