ROLE OF WT1 IN GENITOURINARY DEVELOPMENT AND TUMORIGENESIS

Denys-Drash(DDS)是一种罕见的人类疾病，严重的泌尿生殖系统异常导致肾病、条纹性腺、外阴不清、男性假两性畸形和肾母细胞瘤。这种情况主要影响XY个人。DDS患者在生殖器异常的程度上表现出很大的变异性，一些人同时具有Mullerian和Wolffian派生结构，而另一些人则两者都没有。WT1基因锌指区域的错义突变是DDS发病过程中的一个重要事件。外显子0氨基酸394(R394W)是DDS最显著的突变热点之一，约占DDS患者的40%。WT1的一些肾脏和性腺特异性靶点已经确定，并可能是DDS表型发展的关键成分。为了确定R394W突变是否会导致与人类一样的小鼠DDS表型，将使用PCR定点突变和cre-lox基因靶向策略建立包含密码子394处Arg到Trp替换的小鼠模型。这种突变对生殖道(GU)发育的影响将通过大体形态表型的检查来评估。此外，将通过原位杂交和免疫组织化学方法研究该突变在DDS发生中的作用，通过确定对各种肾脏和性腺发育特异性分子标志物表达的影响。如果突变导致不育、胚胎死亡或两者兼而有之，将产生DDS嵌合动物，以确定突变细胞在泌尿生殖器官发育中的作用。此外，这项研究将有助于确定Wt1的这个点突变是否会导致小鼠的Wilms‘s肿瘤。对肿瘤发展的敏感性，也将在与杂合的p53突变小鼠杂交后进行分析。建立分析DDS的小鼠模型将对确定WT1点突变如何导致DDS患者的一系列泌尿生殖系统影响非常有用。

Denys-Drash (DDS) is a rare human condition in which severe urogenital abnormalities result nephropathy, streak gonads, ambiguous genitalia, male pseudohermaphroditism and Wilms' tumor. The condition affects mostly XY individuals. DDS patients show large variability in the extent of the genital abnormalities some have both Mullerian- and Wolffian-derived structures, and others have neither. Missense mutation in the zinc-finger region of the WT1 gene is a critical event in the elaboration of DDS. One of the most prominent mutational hot spots is amino acid 394 in exon 0 (R394W), which occurs in 40% of the DDS patients. A number of kidney- and gonadal-specific targets of WT1 have identified, and are likely to be critical components in the development of the DDS phenotypes. To determine whether the R394W mutation will cause the DDS phenotypes in mice as seen in human, a mouse model containing an arg to trp substitution at codon 394 will be generated using PCR site-directed mutage4nesis and the cre-lox gene-targeting strategies. The effects of this mutation on genitourinary (GU) development will be assessed by examination of gross morphological phenotypes. In addition, the role of this mutation in the development of DDS will be studied by in situ hybridization and immunohistochemistry by determining the effects on the expression of various kidney- and gonadal-development specific molecular markers. If the mutation results in infertility, embryonic lethality or both, DDS chimeric animals will be generated to determine the contribution of the mutant cells in the development of the urogenital organs. Moreover, this study will help to determine if this point mutation in Wt1 causes Wilms' tumor in mice. Susceptibility to tumor development, also will be analyzed after cross- breeding with heterozygous p53 mutant mice. The establishment of a mouse model to analyze DDS will be extremely useful in determining, how a point mutation in WT1 causes a constellation of urogenital effects in patients with DDS.