CORE--MOLECULAR BIOLOGY

丹娜-法伯癌症研究所建立了分子生物学核心 帮助研究所实验室更容易和成本效益更高的设施 使用分子生物学技术和试剂如寡核苷酸， 合成肽，微蛋白测序，质量分析，DNA 测序、蛋白质配体测定和核酸的电子显微镜检查 核酸和核酸-蛋白质相互作用。 本申请要求 资金，以部分支付收费制度不包括的费用。 的 设施包含以下主要设备：应用生物系统394 DNA/RNA合成仪，Applied Biosystems 380 B DNA合成仪，Applied Biosystems 477蛋白质测序仪，120 A分析仪，Applied Biosystems 430 肽合成仪，Hewlett Packard HP 1090微孔液体 色谱仪，2台沃茨HPLC Applied Biosystems 420/H-130氨基酸 分析仪，Finnigan Lasermat TOF质谱分析仪，Pharmacia BIAcore 生物传感器，Applied Biosystems 373 DNA测序仪，Zeiss EM 10A Electron 显微镜 该设施由一名设施主任和4名全职工作人员组成。 提供不同服务的时间技术人员，以及提供 对希望使用特定仪器的研究者的指导 自己

The Dana-Farber Cancer Institute has established a Molecular Biology Core Facility to help Institute laboratories more easily and cost effectively use molecular biology techniques and reagents such as oligonucleotides, synthetic peptides, microprotein sequencing, mass analysis, DNA sequencing, protein-ligand assays and electron microscopy of nucleic acids and nucleic acid-protein interactions. This application requests funds to partially cover costs not covered by a charge back system. The facility contains the following major equipment: Applied Biosystems 394 DNA/RNA Synthesizer, Applied Biosystems 380B DNA Synthesizer, Applied Biosystems 477 Protein Sequencer, 120A Analyzer, Applied Biosystems 430 Peptide Synthesizer, Hewlett Packard HP1090 Micropore Liquid Chromatograph, 2 Waters HPLC's Applied Biosystems 420/H-130 Amino Acid Analyzer, Finnigan Lasermat TOF Mass Analyzer, Pharmacia BIAcore biosensor, Applied Biosystems 373 DNA Sequencer, Zeiss EM 10A Electron Microscope. The facility is staffed by a facility director and 4 full time technicians who provide the different services as well as provided instruction to investigators who wish to use specific instruments themselves.