CORE--MUTANT RODENT RESOURCE

在前一个赠款期的研究越来越集中在 转基因和突变动物在肿瘤研究中的应用 发展变种啮齿动物资源，一个新的共享服务，将 为麦卡德尔科学家提供操纵基因组的能力 通过提供以下便利， 技术： .原核显微注射法制备转基因小鼠和大鼠 .显微注射胚胎干细胞构建嵌合体小鼠 进入宿主囊胚 .小鼠精子的冷冻保存

Research over the preceding grant period has focused increasingly on the use of transgenic and mutant animals for our studies of neoplastic development. The Mutant Rodent Resource, a new shared service, will provide McArdle scientists with the capacity to manipulate the genome of experimental animals by providing ready access to the following technologies: . Generation of transgenic mice and rats by pronuclear microinjection . Generation of chimeric mice by microinjection of embryonic stem cells into host blastocysts . Cryopreservation of mouse sperm