ROOT SURFACE CARIES PREVENTION AND REMINERALIZATION

最近的流行病学调查表明龋齿有所减少。 在儿童中，强调冠面和牙根表面的问题 老年人口中的衰败。随着更多的牙齿被保留和无牙 下降，成年人口中更多的体表将比以往任何时候都处于危险之中 在此之前。这项提议的总体目标是减少 对龋齿过程(特别是根面龋齿)的影响 当疾病的病因已被弄清时的二级预防。 通过将实验室和临床获得的信息结合起来 并改进了用于模拟龋病过程的模型系统， 理想的根面龋病防治策略应该是 将被澄清。 这项建议打算使用体外和在体模型来描述 疾病进程，并确定最适合的预防药物 用于预防根面龋病。 具体地说，我们打算(1)确定所需的氟化物水平 用矿化表面层形成根表面病变，(2)测试 假设再矿化发生在剩余矿物上而不是 脱钙基质，(3)评价再矿化病变的抵抗力 为了进一步酸蚀，(4)测试氟化物是否存在于 脱矿或再矿化循环是最重要的，(5)确定 现有氟化物制剂抑制根面龋病的能力 在一个原位模型中。 体外pH循环实验及口内冠模型的建立 评估脱矿和再矿化的间歇性阶段 不是连续的。单层切片技术的敏感性 将允许在同一截面上进行前后测量。两者都有 偏振光显微镜和显微放射照相术将用于 定性和定量的分析。这些研究应该确定 当前防氟剂的有用性，从而解决了 新的和/或改良剂和车辆是否 有效预防根面龋齿所需。

Recent epidemiological surveys have shown a reduction in dental caries in children, while highlighting the problem of coronal and root surface decay in elderly populations. As more teeth are retained and edentulism declines, more surfaces will be at risk in the adult population than ever before. The overall objective of this proposal is to reduce the extent of the caries process (in particular root surface caries) by primary and secondary prevention when the etiology of the disease has been clarified. By combining the information obtained in both the laboratory and clinic and refining the model system used to simulate the caries process, the ideal preventive and treatment strategies for root surface caries should be elucidated. This proposal intends to use both in vitro and in situ models to describe the disease process, and to determine the preventive agents most suited for use in root surface caries prevention. Specifically, we intend to (1) determine the level of fluoride needed to form root surface lesions with a mineralized surface layer, (2) test the hypothesis that remineralization occurs on remaining mineral and not demineralized matrix, (3) evaluate the resistance of remineralized lesion to further acid attack, (4) test whether fluoride present in the demineralizing or remineralizing cycle is most important, (5) determine the ability of existing fluoride agents to inhibit root surface caries in an in situ model. In vitro pH cycling experiments as well as the intraoral crown model assess episodic periods of demineralization and remineralization as opposed to continuous. The sensitivity of the single-section technique will allow before and after measurements on the same section. Both polarized light microscopy and microradiography will be used in qualitative and quantitative analyses. These studies should determine the usefulness of current fluoride preventive agents, and thus address the question of whether new and/or modified agents and vehicles are needed to efficiently prevent root surface caries.