RELEASE OF DIAGNOSTIC AND CARIES PREVENTIVE AGENTS

如果修复体和牙齿结构之间的粘结因 修复材料的侵蚀或聚合收缩，如果， 在某些条件下，细菌迁移发生在边缘区域内， 间隙，然后可以开始牙齿结构的脱矿作用。 修复体附近早期脱矿的诊断 仍然是一个主观的任务，当一个微小的差距 沿着牙齿修复界面存在。 X光片通常不会 检测这些复发性病变;目前，没有更好的技术， 比去除修复体和直接目视检查更有效 寻找白色斑点或更严重病变的证据 远程 项目4的目标是开发一个受控释放系统， 环境pH值的变化，可作为 用于龋齿诊断和/或保护的修复程序 牙齿结构与牙弓相邻。 该系统应执行以下任务时，pH值的 环境达到一个临界值：a）信号的存在早期 通过释放临床医生可以 识别; B）通过以下方式抑制该位置的继续定殖 微间隙中的致龋细菌和由此产生的酸分泌; c） 引发脱矿牙本质的再矿化。 我们将应用 微球技术来实现这些目标。 的优点 微球是三重的：1）可以装载各种染料作为标记物， 治疗剂可以储存在微球内; 2）微球 可由各种类型的树脂制成 轮廓;和3）微球可以被制成保持它们的物理特性， 即使在完全释放后也是完整的。 在我们目前的中心赠款期间 期间，我们合成了两个主要系列的pH敏感性聚合物， 释放载体;聚（苯乙烯-co-乙烯基吡啶）和聚（苯乙烯-co-N，N，- 甲基丙烯酸二乙氨基乙酯）。 用于研究的药物 释放机制包括两种染料（9-氨基吖啶和钠 荧光素），矿化增强剂如氟化钠，和 抗菌剂如氯己定和氟离子。 了解聚合物作为控释装置的潜力 并促进递送龋齿标记物的制剂 诊断或生物活性剂的特定部位，我们建议研究 共聚物组成对试剂负载和pH刺激影响 从微球中释放。 通常微球需要结合 一些树脂系统将释放剂释放到特定部位。 的影响 还将测定树脂体系对从微球中释放的影响。 为了提高诊断系统的有效性， 将制备可溶性染料并进行测试。 该项目的最终目标 4是分析作为组成的函数的释放潜力， 以选择适合特定释放速率的理想系统。 使用 将探索在其他应用中合成的聚合物系统。 为 系统的有效性，该版本还将进行测试， 体外和体内的杀菌释放和氟离子释放 细菌培养 在2004年，我们将进行可行性研究， 该诊断系统生成用于未来临床的初步数据， 问题研究

If the bond between the restoration and the tooth structure is broken due to erosion or polymerization shrinkage of restorative materials, and if, under certain conditions, bacterial migration occurs within the marginal gap, then demineralization of tooth structure can be initiated. Diagnosis of such early demineralization adjacent to a restoration remains a subjective task that is complicated further when a microgap exists along the tooth-restoration interface. Radiographs often do not detect these recurrent lesions; currently, no better technique is available than removal of the restoration and direct visual examination for evidence of white sports or more advanced lesions. The long range goal of Project 4 is to develop a controlled-release system driven by the changes in the pH of the environment which can be incorporated as part of the restorative procedure for the caries diagnosis and/or protecting tooth structure adjacent to the restorations. This system should perform the following tasks when the pH of the environment reaches a critical value: a) signal the presence of early demineralization by releasing a distinct marker that the clinician can recognize; b) inhibit the continued colonization of this location by cariogenic bacteria and the resultant acid secretion in the microgap; c) initiate remineralization of the demineralized dentin. We will apply microsphere technology to achieve these goals. The advantages of microspheres are three-fold: 1) one can load various dyes as marker and therapeutic agents can be stored within the microspheres; 2) microspheres can be fabricated from various types of resin to form any desired release profile; and 3) the microspheres can be made to maintain their physical integrity even after complete release. During our current Center grant period, we have synthesized two main series of pH-sensitive plymers as release vehicles; Poly(styrene-co-vinylpridine) and poly(styrene-co-N,N,- diethylaminoethylmethacrylates). Agents which have been used to study release mechanisms include two dyes (9-aminoacridine and sodium flluorescein), mineralization enhancers such as sodium fluoride, and antibacterial agents such as chlorhexidine and fluoride ions. To understand the potential of the polymer as a controlled-release device and to facilitate formulation for delivering markers for caries diagnostic or bioactive agents to specific sites, we propose to study the effect of copolymer composition on agent loading and pH-stimulated release from microspheres. Often the microspheres need to incorporate some resin system to release agents to specific-sites. The effect of the resin system on the release from microspheres will also be determined. To improve the effectiveness of the diagnostic system an non-ionic water soluble dye will be made and tested. The ultimate goal of the Project 4 is to analyze release potential as a function of compositions in order to choose the ideal system for specific release rates. The use of polymer systems synthesized in other applications will be explored. For the effectiveness of the system, the release will also be tested for bactericidal release and fluoride ion release both in vitro and in bacterial culture. At year 04, we will conduct a feasibility study of the diagnostic system to generate preliminary data for future clinical studies.