MOLECULAR MARKERS FOR SQUAMOUS CELL CARCINOMA

鳞状细胞癌（SCC）是最常见的口腔恶性肿瘤， 腔 未能诊断和治疗早期病变，尽管他们很好， 确定的组织病理学标准和临床检测的可及性， 强调了先进的介绍和显着的发病率， 这种癌症患者的死亡率。 由于发展和 肿瘤的进展是由于各种 遗传改变，识别与早期， 中晚期口腔鳞状病变将具有重要的 诊断和临床意义。 p53基因，根据我们的 初步数据，选定的染色体位点，重点是完善的 利用微卫星标记定位3p21、8p21、9p21和11p15.5区域 将对正常和发育异常的显微切割样本进行分析 上皮和侵袭性病变。 第一阶段 在这项研究中，100个回顾性病例的样本将成为我们的材料， 确定每一个最高和最一致的标记， 病理形态学分期 第二阶段，每年20个 前瞻性切除的标本将被仔细和系统地 针对正常的、不同的预处理的多个空间上分离的样本进行映射， 恶性上皮和恶性病变，以确定克隆 所选标志物的进展、稳定性和异质性。 的 结果将与组织病理学进展、侵袭性 肿瘤特点及流行病学因素。

Squamous cell carcinoma (SCC) is the most common malignancy of the oral cavity. Failure to diagnose and treat early lesions, despite their well defined histopathologic criteria and accessibility to clinical detection, underlies the advanced presentation and the significant morbidity and mortality of patients with this cancer. Since the development and progression of neoplasms result from continuous accumulation of various genetic alterations, identifying genetic markers associated with early, intermediate and advanced oral squamous lesions will have important diagnostic and clinical implications. p53 gene and, based on our preliminary data, selected chromosomal loci with emphasis on the refined mapping of 3p21, 8p21, 9p21 and 11p15.5 regions by microsatellite markers will be analyzed on microdissected samples of normal and dysplastic epithelium and invasive lesions from each specimen. In the first phase of the study, samples from 100 retrospective cases will form our materials to determine the highest and most consistent markers for each pathomorphologic stage. In the second phase, each of 20/year prospectively resected specimens will be carefully and systematically mapped for multiple spatially separate samples of normal, different pre- malignant epithelium and malignant lesions to determine clonal progression, stability and heterogeneity of the selected markers. The results will be correlated with histopathologic progression, aggressive tumor characteristics and epidemiological factors.