CORE--CELL, TISSUE AND FLUID BANK, CELL CULTURE AND CYTOGENETICS

华盛顿大学ADRC核心、细胞、组织和液体库细胞 文化和细胞遗传学，是历史上最古老的家族遗传学知识库。 阿尔茨海默氏病（AD）家系。 内核在低温环境下 保存的AD和对照淋巴母细胞样细胞系，成纤维细胞， 淋巴细胞和血浆。 该核心的目标是： A.获取、扩增和冷冻保存各种类型的细胞并冷冻 来自家族性和散发性阿尔茨海默病患者的血浆 本发明涉及用于治疗AD的方法和用于获取和保存细胞、组织和/或细胞系的方法 来自其他退行性疾病患者，以及适当的对照。 B。应研究者要求分发储存的细胞系或菌株 在ADRC，并经ADRC批准，在调查人员 华盛顿大学等机构。 C.对淋巴母细胞样细胞系进行细胞遗传学表征 早发性伏尔加-德国家族性 老年痴呆症 D.支持链接的RO-1，其中一个目的是确定阶段 14号染色体连锁基因与家族性阿尔茨海默病基因的关系。 这将通过进行体细胞杂交融合来实现， 具有LCL的CHO细胞中的补体叶酸途径（腺嘌呤E-）缺乏 从家庭成员。 将产生克隆，并将细胞团块 提供DNA。 E.为了支持项目4，获得增长和维护的专业知识， 小鼠神经胶质细胞和神经元细胞。

The University of Washington ADRC Core, Cell, Tissue and Fluid Bank Cell Culture & Cytogenetics, is historically the oldest repository for familial Alzheimer's Disease (AD) pedigrees. The Core maintains cryogenically preserved AD and control lymphoblastoid cell lines, fibroblasts, lymphocytes, and plasma. The aims of the Core are to: A. Acquire, expand and cryopreserve various cell types and to freeze plasma derived from patients with familial and sporadic Alzheimer's disease (AD) and to acquire and preserve cells, tissues, and/or cell lines from patients with other degenerative disorders, and appropriate controls. B. Distribute stored cell lines or strains upon request by investigators in the ADRC and, upon approval by the ADRC, to investigators at the University of Washington and other institutions. C. Perform cytogenetic characterization of lymphoblastoid cell lines (LCLs) of affected members of early onset, Volga-German familial Alzheimer's disease. D. Support of linked RO-1 of which one aim is to determine phase relationships of chromosome 14 linked genes to familial Alzheimer's gene. This will be accomplished by performing somatic cell hybrid fusions to complement folate pathway (adenine E-) deficiency in CHO cells with LCLs from family members. Clones will be generated and cell pellets will be provided for DNA. E. In support of Project 4, acquire expertise in growth and maintenance of mouse glial and neuronal cells.