NEUROSCIENCE

有证据表明，基底节内多巴胺的释放 神经节显示出昼夜节律模式，并暗示在 基底节功能。将使用免疫细胞化学方法来 多巴胺和EAA受体在大鼠脑内分布的研究 黑。昼夜节律对这些受体分布的影响 在接受褪黑素治疗的动物身上以及在其他动物身上也是如此 缠绕在各种光照条件下。后来， 与运动相关的DA功能的电生理测定 基底节的机制，将被评估。此外， 褪黑素对大脑皮层DA和EAA调节机制的体液效应 黑质将在脑切片中使用常规方法进行研究 电流钳技术以及全细胞膜片钳技术。我们还将 确定每种受体亚型的功能作用 电生理和药理学方法(AIM II)。尝试 将使功能参数与明/暗周期和 褪黑激素水平。我们的发现将(I)有助于进一步 对脑内谷氨酸和多巴胺功能的理解(II)阐明 精选多巴胺和谷氨酸的特性及其可能的作用 受体在正常SNC功能机制中的作用及(III)受体的描述 在中枢神经系统DA神经元功能的昼夜节律或褪黑素控制中的作用。

There is evidence to suggest that the release of dopamine within the basal ganglia exhibits a circadian pattern and suggests a similar pattern in basal ganglia function. Immunocytochemical approaches will be used to study the distribution of dopamine and EAA receptors in the rat substantia nigra. The effects of circadian rhythms on these receptor distributions will be determined in melatonin-treated animals, as well as in others entrained to various lighting conditions. Subsequently, electrophysiological determinations of DA function, relative to motor mechanisms in the basal ganglia, will be evaluated. In addition, the humoral effects of melatonin on DA and EAA modulatory mechanisms in the substantia nigra will be studied in brain slices using conventional current-clamp as well as whole cell patch clamp techniques. We will also identify the functional role of each receptor subtype utilizing electrophysiological and pharmacological approaches (Aim II). Attempts will be made to correlate functional parameters with light/dark cycles and melatonin levels. Our findings will (i) contribute to the further understanding of glutamate and DA functions in the brain (ii) elucidate the identity and possible contribution of select DA and glutamate receptors in mechanisms of normal SNc function and (iii) delineate their role in circadian or melatonin control of CNS DA neuronal function.