OOCYTE CHROMATIN ORGANIZATION IN COLLICULOGENESIS & OOGENESIS IN RHESUS

从氟烷下摘除的卵巢中收集卵泡 7只恒河猴的麻醉。卵泡和卵子直径 用荧光法测定和测定染色质组织 显微镜。卵母细胞和卵泡的相对生长曲线 黄土回归技术表现出一条三相曲线。这个 卵泡与卵母细胞生长和成熟的关系 (核周染色质构型)。染色质 组织的进展与卵子大小有关。就.而言 卵泡大小，卵母细胞首先反应(越过无框核仁 阶段)在最早的鼻腔形成时期，然后 在卵泡长到600米之前，反应保持相对不变 当大多数应答者处于部分边缘阶段时。但它 直到卵泡的直径超过1000米 大多数卵母细胞处于完全边框阶段(减数分裂 称职)。

Follicles were collected from ovaries removed under halothane anaesthesia from 7 rhesus monkeys. Follicle and ovum diameters were measured and chromatin organization was determined by fluorescence microscopy. The relative growth curve of oocytes and follicles using a loess regression technique displayed a triphasic curve. The relationship of follicle and oocyte growth and oocyte maturation (perinucleolar chromatin configuration) was analyzed. Chromatin organization progressed in relation to ovum size. In terms of follicle size, oocytes first respond (past the unrimmed nucleolar stage) during the period of earliest antrum formation, then the response remains relatively the same until the follicle reaches 600 m when the majority of responders are at the partial rim stage. But it is not until the follicle has reached a diameter beyond 1000 m that the majority of oocytes are at the fully rimmed stage (meiotically competent).