COMPARISON OF INTRAVENOUS VERSUS MUCOSAL INOCULATION W/ SIV

重要意义艾滋病毒主要是一种性传播疾病，但 关于病毒变异在性行为中的传播情况，人们知之甚少 联系。对通过性传播的病毒类型的了解 可能会使设计疫苗以防止猪流感传播成为可能 爱滋病毒。目的本研究使用恒河猴/SIV模型 检验特定病毒变种有效的假设 通过静脉接种传播，但不通过阴道接种传播 。结果5只动物大剂量静脉接种 SIVmac251。这是一个由许多病毒组成的病毒库 变种。五只动物经阴道接种了相同的疫苗。 股票。所有的动物都被感染了，我们目前 评估感染动物的病毒变种的性质。 我们将比较感染静脉注射和静脉注射的变种 确定接种途径是否会影响动物的类型 感染恒河猴的病毒。未来的方向，如果 特定的SIV变种在感染动物后负责 阴道接种时，我们会连续传代一批病毒 按顺序经阴道接种恒河猴。那我们就会 从最后一只动物的血浆中产生一种原料，并用于 疫苗挑战研究。病毒变异体；HIV粘膜 传输

Significance HIV is primarily a sexually transmitted disease but little is known about the transmission of viral variants during sexual contact. An understanding of the types of viruses transmitted by sex may make it possible to design vaccines to prevent the transmission of HIV. Objective In this study we used the rhesus macaque/SIV model to test the hypothesis that specific viral variants are efficiently transmitted by intravenous inoculation but not by vaginal inoculation . Results Five animals were inoculated intravenously with a high dose of SIVmac251. This is a virus stock with that consists of many variants. Five animals were inoculated intravaginally with the same stock. All the animals became infected and we are currrently assessing the natuire of the viral variants that infected the animals. We will compare the variants that infected the IV and IVAG inoculated animals to determine if the route of inoculation influences the type of viruses which infect rhesus macaques. Future Directions If specific SIV variants are responsible for infecting animals after vaginal inoculation, we will serially passage a virus stock by intravaginally inoculating rhesus macaques sequentially. Then we will produce a stock from the plasma of the last animal and use that for vaccine challenge studies. KEYWORDS viral variants, HIV mucosal transmission