CHARACTERIZATION OF SHIV INTRANASAL VACCINE STUDY

意义艾滋病毒是一种性传播疾病和疫苗 能够防止性传播艾滋病毒的人应该引起粘膜 生殖道中的免疫反应。目的这项研究应该 确定在远端粘膜部位进行免疫接种是否会导致 阴道抗SIV免疫反应与黏膜佐剂的关系 霍乱毒素(CT)对这个假设是有用的。结果2只恒河猴 用沙眼衣原体与1000ug SIVp55混合口服免疫猕猴。 2只动物口服CT和250微克的SIVp55。 用沙眼衣原体和大剂量抗原免疫的动物变得强壮 对P55的系统免疫反应，包括抗体、T细胞 增殖和T细胞因子对P55的反应。免疫者 小剂量P55免疫动物的反应较弱。 在直肠中发现了显著水平的抗P55 IgA抗体 这些动物的阴道分泌物中没有分泌物。四 用100微克CT和100微克CT混合免疫恒河猴 200ug的SIVp55和2只动物鼻腔免疫10ug的CT 与250微克的SIVP55混合。用10ug CT免疫的动物制作成 对P55的强烈系统免疫反应，包括抗体，T细胞 增殖和T细胞因子对P55的反应。意义重大 在直肠分泌物中发现了抗P55 IgA抗体水平 在这些动物的阴道分泌物中。本研究 证明粘膜佐剂CT对恒河猴有效 猕猴。此外，现在很清楚，口服免疫并不是 一种有效的产生粘膜免疫反应的方法 阴道和鼻腔免疫是一种很好的途径 用于激发阴道免疫力的免疫注射。未来方向测试 其他经鼻途径的疫苗制剂。粘膜；粘膜 免疫、鼻腔免疫、SIV抗原；

Significance HIV is a sexually transmitted disease and a vaccine capable of preventing sexual transmission of HIV should elicit mucosal immune responses in the genital tract. Objective This study should determine if immunzation at distant mucosal sites can result in vaginal anti-SIV immune responses and whether the mucosa adjuvant cholera toxin (CT) was useful for this pupose. Results Two rhesus macaques were orally immunized with CT mixed with 1000ug of SIVp55 and 2 animals were orally immunized with CT mixed with 250ug of SIVp55. The animals immunized with CT and high dose antigen made strong systemic immune responses to P55 including antibody, T cell proliferative and T cell cytokine responses to P55. The immune responses in the animal immunized with low dose P55 were weak. Significant levels of anti-p55 IgA antibodies were found in the rectal secretions but not in the vaginal secretions of these animals. Four rhesus macaques were intranasally immunized with 100ug CT mixed with 200ug of SIVp55 and 2 animals were intranasally immunized with 10ug CT mixed with 250ug of SIVp55. The animals immunized with 10ug CT made strong systemic immune responses to P55 including antibody, T cell proliferative and T cell cytokine responses to P55. Significant levels of anti-p55 IgA antibodies were found in the rectal secretions and in the vaginal secretions of these animals. This study demonstartes that the mucosal adjuvant CT is effective in rhesus macaques. In addition, it is now clear that oral immunization is not an effective method for generating mucosal immune responses in the vagina and that intranasal immunization is an excellent route of immunzartion for elicitng vaginal immunity. Future Directions Test other vaccine preparations by intranasal route. KEYWORDS mucosal immunity, intranasal immunization, SIV antigens;