U MINN OFF SITE LOW VOLTAGE SEM STERECON DVMT: CELL & MICROORGANISM ANTIBODIES

（由NIH GM 23484和R01 GM 49530至S. Erlandsen）。 明尼苏达大学正在使用Sterecon， 免疫标记细胞表面蛋白的三维测量 高分辨率、低温、低电压SEM。 三维测量 在10纳米尺度上的蛋白质与预期的大小一致， 标记分子。 测量已经在表面上进行， 人类淋巴细胞和细菌。 Sterecon还用于 测量各种病原体的表面积和鞭毛长度 原生动物 在共焦LM和医院X射线中的其他应用 断层扫描是预期的。 利斯博士改良了Sterecon 方便使用SEM和共焦图像。 M.马尔科访问巫统， 安装和测试Sterecon，Erlandsen博士两次访问奥尔巴尼， 训练 测试和调试Sterecon应用程序的方法， 开发了因特网上的远程站点。

(Supported in part by NIH GM 23484 and R01 GM 49530 to S. Erlandsen). Sterecon is being used at the University of Minnesota for 3-D measurements of immunolabelled cell-surface proteins imaged by high-resolution, low-temperature, low-voltage SEM. 3-D measurements of proteins on the 10nm scale agree with the expected size of the labeled molecules. Measurements have been made on the surfaces of human lymphocytes and bacteria. Sterecon is also being used to measure surface area and flagella length of various pathogenic protozoa. Additional applications in confocal LM and hospital X-ray tomography are anticipated. Dr. Leith modified Sterecon for more convenient use with SEM and confocal images. M. Marko visited UMN to install and test Sterecon, and Dr. Erlandsen visited Albany twice for training. A method for testing and debugging Sterecon applications at remote sites over the Internet was developed.