STRUCTURE & DYNAMICS OF CENTROSOME

(部分由CNRS向M.Bornens提供的赠款支持)这是 正在进行的对人类中心体结构的研究。因为 中心体非常小(0.25×0.5(M))，而且很复杂，只有 研究它们的形态的方法是用EM。正常情况下，中心体 在常规情况下植入和观察后在EM中进行检查 塑料部分。可获得分辨率在一定程度上受到使用 这项技术。我们的实验室已经分离出人类中心体，我们有 在阴性染色后一直在研究它们。我们已经获得了 显示中心粒周期性的高分辨率图像 结构。为了研究3D结构，我们需要自动化的IVEM 层析成像系统由于整体安装的厚度 负性染料的制备及剂量敏感性研究 准备工作。我们还考虑了未来冰冻水合物的工作。 在她的访问中，Ruiz博士记录了六个双倾斜系列和一个 单倾斜层析成像系列。一个测试重建是由Dr。 而是。该项目将在Dr. 雷德马赫。

(Supported in part by CNRS grants to M. Bornens) This is part of an ongoing study of the structure of the human centrosome. Because centrosomes are extremely small (0.25 by 0.5 (m) and complex, the only way their morphology can be studied is with EM. Normally, centrosomes are examined in the EM after embedding and observation in conventional plastic sections. The resolution obtainable is somewhat limited using this technique. Our lab has isolated human centrosomes, and we have been studying them after negative staining. We have obtained high-resolution images showing the periodicity of the centriolar structure. To study the 3-D structure, we needed the IVEM automated tomography system because of the thickness of the whole-mount preparations and the dose-sensitivity of the negative-stain preparations. We also contemplate frozen-hydrated work in the future. On her visit Dr. Ruiz recorded six double-tilt series and one single-tilt tomographic series. A test reconstruction was made by Dr. Rath. This project will continue with the assistance of Dr. Radermacher.