SYNCHROTRON CRYSTALLOGRAPHY OF RNA 3 TERMINAL PHOSPHATE CYCLASE

RNA3‘-末端磷酸环化酶催化ATP依赖性 将3‘-磷酸转化为2’，3‘-环磷酸二酯 RNA的末尾。它是一种必不可少的酶，在所有生物中都是保守的。 王国。收集了RNA3‘磷酸酯晶体的数据 环化酶在含有10 mM AMP的溶液中浸泡。数据收集 统计数据：3A分辨率下97%的完整性和R因子(20A -3A)为5.7。不幸的是，AMP似乎没有被绑定 有可检测到的入住率。

RNA 3'-terminal phosphate cyclase catalyzes the ATP-dependent conversion of the 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. It is an essential enzyme and conserved across all kingdoms. Data were collected for a crystal of RNA 3' Phosphate cyclase soaked in the solution containing 10mM AMP. Data collection statistics: 97% completeness at 3 A resolution and the R-factor (20 A - 3A) is 5.7. Unfortunately, it does not appear that AMP was bound with detectable occupancy.