NUCLEIC ACID FOOTPRINTING OF INTERACTION OF TATA BINDING PROTEIN (TBP) WITH DNA

TBP是核蛋白复合体的一种成分，它是 三种真核生物核糖核酸各自启动转录 聚合酶。TATA结合蛋白(TBP)与 被称为TATA盒的特定启动子序列是 启动由RNA聚合酶转录的基因转录II。 TATA盒的核苷酸序列在不同的自然界中是不同的 正在发生的推动者。同步加速器x射线核技术的应用 酸和蛋白质对蛋白质-DNA相互作用的足迹正在 通过TATA结合蛋白(TBP)与 特异性启动子序列。这种蛋白质的不同寻常之处在于它 在结构中引起戏剧性的和剧烈的构象变化 关于DNA；特别有趣的是在 通过插入两对苯丙氨酸残基，使DNA脱氧核糖核酸。 基于其他类型的动力学数据，HIT也被提出 这些Hintercalation事件可以按顺序进行，并且其 速率可能取决于HBe DNA序列。滴定实验已经被 成功地进行了证明精确同步加速器 该系统可获取人体足迹数据。舞台现在到了 用于启动同步加速器足迹动力学的HSET 他的实验将批判性地检验这一假说。

TBP is a component of the nucleo-protein complexes required for the initiation of transcription by each of the three eukaryotic RNA polymerases. The binding of the TATA Binding Protein (TBP) to specific promoter sequences called TATA boxes is a key step in the initiation of transcription of genes transcribed by RNA polymerase II. The nucleotide se-quence of TATA boxes is variable among naturally occurring promoters. The application of synchrotron x-ray nucleic acid and protein footprinting to protein-DNA interactions is being addressed through the binding of the TATA Binding Protein (TBP) to specific promoter se-quences. This protein is unusual in that it induces a dramatic and drastic conformational change in the structure of the DNA; of particular interest is the introduction of two kinks in Hthe DNA by the intercalation of two pairs of phenylalanine residues. HIt has been proposed based on other types of kinetics data, that these Hintercalation events may proceed sequentially and that their rates may Hbe DNA sequence dependent. Titration experiments have been Hsuc-cessfully conducted which demonstrate that accurate synchrotron Hfootprinting data can be acquired for this system. The stage is now Hset for the initiation of synchrotron footprinting ki-netics Hexperiments that will critically test this hypothesis.