DIFFRACTION ANALYSIS OF FLP RECOMBINASE & ANIMAL LECTINS

在过去的一年里，我们完成了对 C3d是CR2受体的C3片段和配体(见下文科学)。 通过从数据中在Se边缘进行MAD相移来求解该结构 在国际象棋中收集的。该结构含有8个硒原子和In 与大卫·史密斯博士合作，这些数据被用于 说明SNB法测定硒的实用性 下部结构。该结构为C3的激活提供了一个模型 以及对与CR2交互的基础的洞察 受体。我们还完成了对 人半乳糖凝集素-3在乳糖和乳糖络合物中的CRD 从国际象棋收集的数据中提取N-乙酰乳糖胺。这些分子是 在调节细胞-细胞和细胞-基质相互作用方面很重要。我们的 结构定义了碳水化合物结合的基础，并且在 添加提供了对齐聚模式的洞察，如 这种凝集素。此外，我们在国际象棋比赛中收集了关于C型的数据 凝集素和Cre重组酶-DNA复合体的晶体。在这两种情况下 这些结构的解决方案正在进行中。

In the past year we completed the refinement and publication of C3d a C3 fragment and ligand for the CR2 receptor (see Science below). The structure was solved by MAD phasing at the Se edge from data collected at CHESS. The structure contains 8 selenium atoms and in collaboration with with Dr. David Smith, the data was used to illustrate the utility of the SnB method for determining the selenium substructure. The structure provides a model for the activation of C3 as well as insight into the basis for interaction with the CR2 receptor. We have also completed the refinement and publication of the CRD of human galectin-3 in complex with lactose and N-Acetylactosamine from data collected at CHESS. These molecules are important in modulating cell-cell and cell-matrix interactions. Our structure has defined the basis for carbohydrate-binding, and in addition provided insight into the mode of oligomerization, shown by this lectin. In addition, we collected data at CHESS on a C-type lectin and crystals of a CRE recombinase-DNA complex. In both cases the solution of these structures is in progress.