ANTIMITOTIC DRUGS & MICROTUBULE MEDIATED SIGNAL TRANSDUCTION

有证据表明，抗原肽结合到 分子伴侣蛋白，并通过这些转运到MHC I类。 伴侣蛋白 需要更多的实验结果来建立 详细机制 我们将使用基于质谱的技术， 分析伴侣蛋白GRP 94/肽复合物和肽 与GRP 94分离以应对这一挑战。 的目标 本研究课题为：1. 获得第一手证据， 抗原肽与GRP 94复合。 2. 确定 这些抗原肽的序列。 3. 确定哪些地点 抗原肽被结合。 初步结果显示， 质谱法成功测定从GRP中分离的肽序列 94 HPLC 一个肽已通过MALDI/PSD MS测序。 工作将有望实现我们的目标。

There is evidence indicating that antigenic peptides are bound to chaperonin proteins and transported to the MHC class I by these chaperonins. More experimental results are needed to establish the detailed mechanism. We will use mass spectrometry based techniques to analyze the chaperonin protein GRP 94/peptides complex and peptides isolated from GRP 94 to address this challenge. The objectives of this research project are: 1. Obtain first-hand evidence that antigenic peptides are complexed with GRP 94. 2. Determine the sequence of these antigenic peptides. 3. Determine the sites where antigenic peptides are bound. Preliminary results have shown the success of mass spectrometry in sequencing peptides isolated from GRP 94 by HPLC. One peptide has been sequenced by MALDI/PSD MS. Further work will hopefully fulfill our goal.