课题基金 / 基金详情

SIMULTANEOUS 5 HT & PH IMAGING IN PARAFOLLICULAR CELL GRANULES

SIMULTANEOUS 5 HT & PH IMAGING IN PARAFOLLICULAR CELL GRANULES
同时 5 HT
批准号:
6121888
负责人:
REBECCA M WILLIAMS
金额:
$2.24万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-09-30 至 1999-08-31

项目摘要

项目成果

REBECCA M WILLIAMS的其他基金

相关文献

中文摘要
翻译
动脉瘤旁(滤泡旁细胞),发现于甲状腺, 是内分泌细胞,但在发育上与神经元和 而且可以很容易地以纯形式分离出来进行实验 分析。同样,5-羟色胺能神经元也不能被分离。工作 对此细胞的制备显示,分泌能力强 细胞后颗粒内部出现一过性pH下降 刺激。受蛋白激酶调控的氯离子通道 C和G都有牵连。我们同时成像了5-羟色胺和一个 颗粒pH标记物用于研究5-羟色胺的相关性 释放和颗粒剂的pH值。
英文摘要
Paraneurons (parafollicular cells), found in the thyroid gland, are endocrine cells, but developmentally related to neurons and moreover can be readily isolated in a pure form for experimental analysis. Serotoninergic neurons cannot similarly be isolated. Work on this cell preparation has revealed that the secretion competent granule interiors experience transient pH drops after cell stimulation. Chloride channels that are regulated by protein kinases C and G are implicated. We are simultaneously imaging 5-HT and a granule pH marker to investigate proposed correlations between 5-HT release and granule pH.
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