CONFOCAL RAMAN MICROSPECTROSCOPY FOR BIOLOGICAL MICROSTRUCTURE ANALYSIS

我们增强了我们的主体近红外拉曼光谱 共焦显微镜照明和拉曼信号的采集 空间分辨率高达1量级的薄层切片 美光。新颖的光学设计可实现散装和散装之间的切换 显微镜光谱研究相当容易。我们用过这个 用于采集多种光谱的共焦拉曼显微镜系统 动脉、乳房和脑组织的形态结构。这个 最终目标是利用这些信息来理解 块状组织拉曼信号的显微和形态基础 并与组织病理学相关。

We have augmented our bulk near IR Raman spectroscopy with confocal microscope illumination and collection of Raman signals from thin sections with very high spatial resolution on the order of 1 micron. The novel optical design enables switching between bulk and microscope spectroscopic studies fairly easy. We have used this confocal Raman microscope system for collecting spectra of a variety of morphological structures in artery, breast, and brain tissues. The ultimate goal is to use these information for the understanding of microscopic and morphological basis of Raman signals from bulk tissues and correlate with histopathology.