SALT DEPENDENCE OF PROCESSIVITY OF T45G RIBONUCLEASE

苏氨酸45对甘氨酸加工性的盐依赖性 核糖核酸酶A的(T45G)突变将使用31P核磁共振进行检测 T45G作用于多聚腺苷(POLIA)的加工性测定 [S.B.delCarday r R.T.Raines，生物化学33,6031-6037(1994)]。这个 核磁共振分析允许直接确定加工性 在各种盐条件下，T45G的参数pI。从 PI的变化随着盐浓度的变化而变化，可以评估 正极之间库仑相互作用的重要性 带负电荷的磷酸盐在T45G上的带电碱性残基 关于这一系统中的过程性的波利亚。

The salt dependence of processivity of the Threonine 45 to Glycine (T45G) mutation of Ribonuclease A will be determined using a 31P NMR based assay for processivity of T45G acting on Polyadenosine (PolyA) [S.B. delCardayr R.T. Raines, Biochemistry 33, 6031-6037 (1994)]. The NMR assay allows for the direct determination of the processivity parameter, PI, of T45G at a variety of salt conditions. From the changes in PI with the changes in salt concentration, one can assess the importance of the coulombic interactions between the positively charged basic residues on T45G with the negatively charged phosphates on PolyA on processivity in this system.