STEM MASS MAPPING & HEAVY ATOM LABELING OF BIOMOLECULES

布鲁克海文STEM(扫描电子显微镜)资源 为质量和重原子团簇映射提供独特的服务 单个生物分子。主干I将继续定位Heavy 原子团簇和投影质量分布的映射，给出1-2 nm 有用的空间分辨率和2-10%的质量分辨率 因为病毒是以低剂量成像的。第三号干道即将完工， 提高了分辨率、样本处理和数据处理能力 收集。 我们计划继续扩展我们的高效用户/协作者计划 作为特定部位的标记、标本制备、成像和成像 分析方法得到了发展。目前有65个项目在该地区开展。 蛋白质和蛋白质复合体、细丝和丝状蛋白质， 膜和膜蛋白、DNA、RNA和核酸/蛋白质 复合体、病毒蛋白和病毒、重原子团簇和低温 电子显微镜。预计所有项目都将继续导致 在最高质量的期刊上发表文章。 核心研发计划的主要重点将继续是数量 对样品制备的理解以及改进的发展 重原子团簇标签、改进的显微镜、改进的成像 技术(包括使用比能量的低剂量元素测绘 损失)和改进的图像分析方法。

The Brookhaven STEM (Scanning Transmission Electron Microscopy) Resource provides a unique service for mass and heavy atom cluster mapping of individual biological molecules. STEM I will continue localizing heavy atom clusters and mapping projected mass distribution, giving 1-2 nM useful spatial resolution and 2-10% mass resolution for objects as large as viruses imaged at low dose. STEM III is nearing completion with improved capabilities in resolution, specimen handling and data collection. We propose to continue to expand our productive user/collaborator program as site-specific labeling, specimen preparation, imaging and image analysis methods develop. Currently 65 projects are active in the areas of: protein and protein complexes, filaments and filamentous proteins, membranes and membrane proteins, DNA, RNA and nucleic acid/protein complexes, viral proteins and viruses, heavy atom cluster and cryo electron microscopy. All projects are expected to continue to lead to publications in the highest quality journals. Major emphasis of the core R&D program will continue to be quantitative understanding of specimen preparation as well as development of improved heavy atom cluster labels, improved microscopes, improved imaging techniques (including low dose elemental mapping using specific energy losses) and improved image analysis methods.