CONTROL OF GENE EXPRESSION IN CELLULAR SENESCENCE

本计划项目赠款的长期目标是了解 人类细胞衰老的基本分子机制以及 永生化的逆转现象。 具体目标是： 1)使用不同的模型将五个独立的实验室聚集在一起 细胞衰老系统：正常人成纤维细胞，肾上腺皮质 细胞和黑色素细胞以及各种永生的人类细胞系; 在类似的目标下，使用共同的技术和方法， 回答有关分子和细胞机制的问题 在衰老和永生中。 这些研究将审查 在体外细胞中发生的基因表达变化， 在体内，并将建立差异和相似之处， 导致细胞增殖抑制的途径。 2)提供所有项目所需的核心设施， 从而整合资源，这将有利于研究在很大程度上 更低的成本 从而实现方案项目的目标， 鼓励成功的互动， 共同资源。 3)提供正式机制，通过联合研究促进研究互动 策划会议。

The long-range goal of this program project grant is to understand the basic molecular mechanisms involved in human cellular senescence and the reverse phenomenon of immortalization. The specific aims are to: 1) Bring together five individual laboratories using disparate model systems of cellular senescence: normal human fibroblasts, adrenocortical cells and melanocytes and various immortal human cell lines; to work together on similar goals, using common techniques and approaches to answer questions regarding the molecular and cellular mechanisms involved in senescence and immortalization. These studies will examine the changes in gene expression that occur in cells as they are in vitro and in vivo, and will establish the differences and similarities in the pathways that lead to inhibition of cell proliferation. 2) Provide core facilities that are requisite for all the projects and thereby consolidate resources that will benefit the research at a much lower cost. The aims of a program project will thereby be met, encouraging successful interactions and enhancing the utilization of common resources. 3) Provide a formal mechanism to promote research interactions via joint planning meetings.