ADVERSE EFFECTS OF OXIDATIVE STRESS DURING PREGNANCY

工作总结：氧化损伤已被 与几种疾病的病理学有关，但只是最近 是否有证据表明， 条件许多环境和职业暴露是 已知的促氧化剂（例如，许多溶剂和金属），并且它们可以 通过引起氧化损伤对生殖产生不利影响。这 一项研究项目探讨了环境与 暴露、氧化应激和不良生殖条件。 我们有两个主要目标。首先是对数据的分析， 将解决标记物变异量的试点研究 抗氧化剂和氧化剂的状态，由于季节性和 个体内变异和储存程序，以及 比较妊娠和非妊娠女性的结果。结果 到目前为止，表明样品不能储存超过一个 几个小时不改变谷胱甘肽和脂质的水平 过氧化物显著。今年我们完成了一篇论文， 脂质过氧化物的日常可靠性较高， 这个标记在孕妇身上。没有有力的证据表明 我们的样本中的季节性变化。第二个目标是与 实验室科学家设计了一项研究， 氧化状态是否适合流行病学研究。这项工作是 去年秋天在NIEHS举行的国际会议的延续 他们调查了氧化应激标记物领域。此外该 对保加利亚现有数据的小样本分析， 工业污染物，氧化损伤的标志物， 孕妇及其婴儿的生殖结局 继续。

Summary of Work: Oxidative damage has been implicated in the pathology of several diseases, but only recently has it been suggested to have a role in adverse reproductive conditions. Many environmental and occupational exposures are known pro-oxidants (e.g., many solvents and metals), and they may adversely affect reproduction by causing oxidative damage. This research project explores the relationship between environmental exposures, oxidative stress, and adverse reproductive conditions. We working with two major aims. First is analysis of data from a pilot study that will resolve the amount of variability in the markers of anti-oxidant and oxidant status due to seasonal and intra-individual variation and to storage procedures, and to compare the results in pregnant and non-pregnant women. Findings to date indicate that samples cannot be stored at all for more than a few hours without altering the level of glutathione and lipid peroxides significantly. This year we finished a paper indicating high day-to-day reliability in lipid peroxides and higher values of this marker in pregnant women. There was no strong evidence of seasonal variability in our sample. A second aim is to work with laboratory scientists in designing a study to evaluate mamkers of oxidative status for suitability in epidemiologic studies. This work is a continuation of an international meeting held last fall at NIEHS that surveyed the field of oxidative stress markers. In addition, the analysis of small samples of existing data from Bulgaria concerning industrial pollutants, markers of oxidative damage, and reproductive outcome in pregnant women and their infants continues.