GIGAPOROUS PARTICLES FOR PREPARATIVE SEPARATIONS

描述：(申请人描述)Fenris Technology Research，Inc. 建议开发用于固定物的巨孔聚合物颗粒 用于制备液相色谱的相。它们将被设计成包含 大孔，以允许流动相以一种速度流过颗粒 速度快到足以使固定相中的对流传质超过 扩散传质。在这种情况下，板的高度不会增加 随着流速的提高，可以在 高流速。这对于制备性分离尤为重要。 蛋白质和其他具有小扩散系数的大分子。这一阶段 I的目的是证明制备聚合物是可行的 已知的高孔隙率的颗粒，并且可以控制和改变 通过适当的配方选择来提高孔隙率。机械和渗透性 这些粒子的性质将被研究。 建议的商业应用： 这项研究计划中准备的材料将用作固定相。 用于制备层析法。它们特别适合于纯化蛋白质。 和其他高相对分子质量的生物分子。

DESCRIPTION: (Applicant's description) The Fenris Technology Research, Inc., proposes to develop gigaporous polymer particles to be used as stationary phases for preparative liquid chromatography. They will be designed to contain large pores in order to allow mobile phase to flow through the particles at a rate fast enough for convective mass transfer in the stationary phase to exceed diffusive mass transfer. Under these conditions, plate height does not increase with flow velocity making it possible to conduct efficient separations at a high flow rate. This is particularly important for preparative separations of proteins and other macromolecules with small diffusion coefficients. The Phase I objectives are to demonstrate that it is feasible to prepare polymer particles of known high porosity and is possible to control and vary the porosity by appropriate choice of formulation. The mechanical and permeability properties of these particles will be investigated. PROPOSED COMMERCIAL APPLICATION: The materials to be prepared in this research program will be useful as stationary phases for preparative chromatography. They are particularly well-suited for purifying proteins and other high molecular weight biomolecules.