OLDEST-OLD MORTALITY AND DISABILITY FOR DROSOPHILA

我们建议调查#年最高年龄的死亡率和发病率。 果蝇，一种非常适合于 实验室分析和基因操作。 具体目标是： 1)QTL定位-我们将估计数量、染色体位置和 导致寿命变化的基因的影响大小 基于DNA的遗传标记。 2)基因人口学--我们将估算与年龄相关的死亡率 遗传携带的小染色体片段的参数 同质重组自交系。 3)ADL实验-我们将在 遗传同质性果蝇的队列，并估计遗传 不同基因型别的死亡率和发病率的相关性。 显著的特点是：(一)实验强调大范围的数据 用于估计遗传和人口统计参数的收集。(Ii)QTL 测绘实验使用了DNA中的一些最新进展 技术(聚合酶链式反应和限制性片段长度多态性)。(3)数据将提供有关 雌雄相关的遗传学基础。(4)数据将被存档，用于 一般访问权限。 健康相关性：这项研究将提供关于 模型中年龄最大的发病率和死亡率的可塑性 实验系统。

We propose to investigate mortality and morbidity at the oldest ages in Drosophila melanogaster, an experimental system that is well-suited to laboratory analysis and genetic manipulation. Specific aims are: 1) QTL Mapping- We will estimate the number, chromosomal location, and magnitude of effects of genes that cause variation in longevity using DNA-based genetic markers. 2) Demography of Genes- We will estimate age-dependent mortality parameters for small chromosomal segments carried in genetically homogeneous recombinant inbred lines. 3) ADL Experiments- We will characterize age-dependent disability in cohorts of genetically homogeneous flies, and estimate the genetic correlation of mortality and morbidity across genotypes. Notable features are: (i) The experiments emphasize large scale data collection for estimation of genetic and demographic parameters. (ii) QTL mapping experiments employ some of the most recent developments in DNA technology (PCR and RAPDs). (iii) Data will be informative about the genetic basic of male-female correlations. (iv) Data will be archive for general access. Health relevance: The research will provide information about the plasticity of morbidity and mortality at the oldest ages in a model experimental system.