CONTROL OF GENE EXPRESSION IN CELLULAR SENESCENCE

这项计划项目拨款的长期目标是了解 人类细胞衰老的基本分子机制 逆转不朽现象。 具体目标是： 1)使用不同的模式将五个独立的实验室聚集在一起 细胞衰老系统：正常人肾上腺皮质成纤维细胞 细胞和黑素细胞以及各种不朽的人类细胞系；工作 共同实现相似的目标，使用共同的技术和方法来 回答有关分子和细胞机制的问题 在衰老和永生中。这些研究将考察 在细胞中发生的基因表达的变化，如它们在体外和 在体内，并将建立不同和相似之处 导致抑制细胞增殖的途径。 2)提供所有项目所必需的核心设施 从而在很大程度上整合对研究有利的资源 更低的成本。从而将实现计划项目的目标， 鼓励成功的互动并提高对 公共资源。 3)提供正式机制，通过联合促进研究互动 计划会议。

The long-range goal of this program project grant is to understand the basic molecular mechanisms involved in human cellular senescence and the reverse phenomenon of immortalization. The specific aims are to: 1) Bring together five individual laboratories using disparate model systems of cellular senescence: normal human fibroblasts, adrenocortical cells and melanocytes and various immortal human cell lines; to work together on similar goals, using common techniques and approaches to answer questions regarding the molecular and cellular mechanisms involved in senescence and immortalization. These studies will examine the changes in gene expression that occur in cells as they are in vitro and in vivo, and will establish the differences and similarities in the pathways that lead to inhibition of cell proliferation. 2) Provide core facilities that are requisite for all the projects and thereby consolidate resources that will benefit the research at a much lower cost. The aims of a program project will thereby be met, encouraging successful interactions and enhancing the utilization of common resources. 3) Provide a formal mechanism to promote research interactions via joint planning meetings.