CORE--CELL, TISSUE AND FLUID BANK, CELL CULTURE AND CYTOGENETICS

华盛顿大学ADRC核心、细胞、组织和流体库细胞 文化与细胞遗传学，历史上是最古老的家族遗传库 阿尔茨海默病(AD)家系。核心保持低温状态 保存的AD和对照淋巴母细胞系、成纤维细胞、 淋巴细胞和血浆。 核心计划的目标是： A.获取、扩增和冷冻保存各种类型的细胞并冷冻 家族性和散发性阿尔茨海默病患者的血浆 疾病(AD)以及获取和保存细胞、组织和/或细胞系 来自其他退行性疾病的患者，并进行适当的对照。 B.应调查人员的要求分发储存的细胞系或菌株 在ADRC，并在得到ADRC批准后，提交给 华盛顿大学和其他机构。 C.对淋巴母细胞系进行细胞遗传学鉴定 受影响的早发性成员(LCLS)，伏尔加-德国家族 阿尔茨海默氏症。 D.支持连接的RO-1，其中一个目标是确定阶段 14号染色体连锁基因与家族性阿尔茨海默病基因的关系。 这将通过进行体细胞杂交来实现 LCLS CHO细胞的补体叶酸途径(腺嘌呤E-)缺乏 从家庭成员那里。克隆将被生成，细胞颗粒将被 提供了DNA。 E.为支持项目4，获得增长和维护方面的专业知识 小鼠神经胶质细胞和神经细胞。

The University of Washington ADRC Core, Cell, Tissue and Fluid Bank Cell Culture & Cytogenetics, is historically the oldest repository for familial Alzheimer's Disease (AD) pedigrees. The Core maintains cryogenically preserved AD and control lymphoblastoid cell lines, fibroblasts, lymphocytes, and plasma. The aims of the Core are to: A. Acquire, expand and cryopreserve various cell types and to freeze plasma derived from patients with familial and sporadic Alzheimer's disease (AD) and to acquire and preserve cells, tissues, and/or cell lines from patients with other degenerative disorders, and appropriate controls. B. Distribute stored cell lines or strains upon request by investigators in the ADRC and, upon approval by the ADRC, to investigators at the University of Washington and other institutions. C. Perform cytogenetic characterization of lymphoblastoid cell lines (LCLs) of affected members of early onset, Volga-German familial Alzheimer's disease. D. Support of linked RO-1 of which one aim is to determine phase relationships of chromosome 14 linked genes to familial Alzheimer's gene. This will be accomplished by performing somatic cell hybrid fusions to complement folate pathway (adenine E-) deficiency in CHO cells with LCLs from family members. Clones will be generated and cell pellets will be provided for DNA. E. In support of Project 4, acquire expertise in growth and maintenance of mouse glial and neuronal cells.