CORE--QUANTITATIVE MOLECULAR ANALYSIS

定量分子分析核心将支持创新、创新 最新的荧光检测和动力学聚合酶链式反应技术 ABI 7700序列检测器机器能够灵敏、具体和 重复性定量血液和组织中的靶DNA和RNA序列 具有高吞吐量。因此，使他们能够很容易地 量化一系列适用于其基因和基因转录本 特定的研究领域。此外，该核心将提供 靶序列严格匹配引物的构建 用于扩增和构建序列特异性荧光- 用于靶序列检测的连接寡核苷酸。量化的 分子分析核心将由纳贝尔博士和沃林斯基博士共同指导 分别是密歇根大学和西北大学。Dr。 纳贝尔和他在密歇根大学的工作人员贝蒂·吴博士， 沃林斯基博士、克拉克·布朗博士和帕特里夏·奥托女士，他的工作人员 西北大学，将负责监督，详细， 标本处理、DNA和RNA加工及寡核苷酸引物 并进行了符合严格要求的探头顺序设计和施工 对化验的严格要求。DNA测序核心的目标是 是：(1)提供一个高通量、可重复性的核心实验室 定量DNA和RNA分析；(2)为构建 用于靶序列扩增的严格匹配的引物；和(3) 为序列特异性荧光连接的构建提供了条件 用于靶序列检测的寡核苷酸。定量分子 分析核心促进了研究人员之间的互动，允许 研究人员可以方便地访问自动化的定量分子分析， 降低了个人研究人员使用这项技术的成本， 促进新项目的开展。

The QUANTITATIVE MOLECULAR ANALYSIS CORE will support novel, innovate state-of-the-art fluorescence detection and kinetic PCR technology using the ABI 7700 Sequence Detector machine to sensitively, specifically and reproducibly quantify target DNA and RNA sequences in blood and tissue with high through put. Thus, making it possible for them to readily quantify a range of genes and gene transcripts applicable to their particular area of research. Additionally, the core will provide for the construction of stringency matched primers for target sequence amplification and for the construction of sequence-specific fluorescence- linked oligonucleotides for target sequence detection. The quantitative molecular analysis core will be co-directed by Drs. Nabel and Wolinsky of the University of Michigan and Northwestern University, respectively. Dr. Nabel and Dr. Betty Wu, his staff member at the University of Michigan, and Dr. Wolinsky and Dr. Clark Brown and Ms. Patricia Otto, his staff at Northwestern University, will be responsible for overseeing, in detail, the specimen handling, DNA and RNA processing and oligonucleotide primer and probe sequence design and construction that conforms to the strict stringency requirements of the assay. The goal of the DNA sequencing core is to: (1) provide a core laboratory of high through put, reproducible quantitative DNA and RNA analysis; (2) provide for the construction of stringency matched primers for target sequence amplification; and (3) provide for the construction of sequence-specific fluorescence-linked oligonucleotides for target sequence detection. The quantitative molecular analysis core facilitates interactions among researchers, allows researchers to readily access automated quantitative molecular analysis, lowers the cost of this technology to individual researchers, and facilitates the undertaking of new projects.