OPIOID IN RNAS AND SEROTONERGIC RECEPTORS IN HIPPOCAMPUS OF EPILEPTIC PATIENTS

很少有研究检测患者海马区的脑化学成分。 患有顽固性癫痫。这在一定程度上是由于 获取适合这些类型的新鲜脑组织样本 学习。这项研究项目的跨学科方法使它 非常独特。研究小组由一名神经外科医生(Inserni博士)组成。 对基础研究感兴趣的人，神经内分泌学家(塞加拉博士) 具有受体结合、放射免疫分析和图像分析方面的专业知识 和一位生化神经学家(安古洛博士)，他在 原位杂交、图像分析和受体结合技术。 这项研究将检查切除的脑组织，特别是海马体。 来自难治性复杂的颞叶部分性癫痫患者 起源。5-羟色胺能系统和阿片系统将进行原位研究。 杂交和受体放射自显影以确定这些系统 参与了颞叶癫痫患者的病因学 医学上难以治愈的癫痫。目标：这项研究将提供基本的 关于人类海马区5-羟色胺能和阿片系统的知识。在……里面 此外，它还将比较癫痫患者和非癫痫患者的海马体 患者确定海马区内源性阿片类药物是否发生变化 5-羟色胺能系统与颞叶的病因学有关 难治性癫痫患者的癫痫。具体目标： (L)确定内源性阿片合成的部位(脑啡肽和 强啡肽)在人的海马区。(2)调查患者是否患有 起源于颞叶的难治性复杂部分性癫痫 内源性阿片类药物水平的永久性变化。(三)确定 5-羟色胺能1A和1B受体亚型的分布和密度 在人脑的海马体中。(4)调查复杂的部分 人类起源于颞叶的癫痫发作与脑电变化有关 海马区5-羟色胺能受体密度。(5)确定是否发生变化 内源性阿片类药物水平或5-羟色胺能受体密度相关 难治性脑脊髓炎患者的海马区硬化程度 起源于颞叶的复杂部分性癫痫。

Very few studies have examined brain chemistry in hippocampi of patients with intractable epilepsy. This is due in part to the difficulty in obtaining fresh brain samples that are suitable for these types of studies. The interdisciplinary approach to this research project makes it quite unique. The research team consists of a neurosurgeon (Dr. Inserni) who is interested in basic research, a neuroendocrinologist (Dr. Segarra) with expertise in receptor binding, radioimmunoassay and image analysis and a biochemical neuroscientist (Dr. Angulo) with expertise in the technique of in situ hybridization, image analysis and receptor binding. This study will examine brain tissue, specifically hippocampus, excised from patients with intractable complex partial seizures of temporal lobe origin. The serotonergic and opiate systems will be studied by in situ hybridization and receptor autoradiography to determine if these systems are involved in the etiology of temporal lobe epilepsy in patients with medically intractable seizures. Objective: This study will provide basic knowledge on the serotonergic and opiate system of human hippocampus. In addition, it will Compare the hippocampus of epileptic vs non-epileptic patients to determine if changes in the hippocampal endogenous opiate and serotonergic systems are implicated in the etiology of temporal lobe epilepsy in patients with medically intractable seizures. Specific Aims: (l) To determine the sites of endogenous opiate synthesis (enkephalins and dynorphins) in human hippocampi. (2) To investigate if patients with intractable complex partial seizures of temporal lobe origin exhibit permanent changes in the levels of endogenous opiates. (3) To determine the distribution and density of serotonergic 1A and 1B receptor subtypes in the hippocampi of human brains. (4) To investigate if complex partial seizures of temporal lobe origin in humans is associated with changes in hippocampal serotonergic receptor density. (5) To determine if changes in endogenous opiates levels or serotonergic receptor density is correlated with the degree of hippocampal sclerosis in patients with intractable complex partial seizures of temporal lobe origin.