DEVELOPMENT OF THE SENSE OF TASTE

描述：(改编自申请者摘要)：本提案涉及 几种神经营养因子在胎儿发育中的作用 支配味蕾的感觉神经元，以及胎儿之间的相互作用 成熟味觉器官形态发生中的细胞类型。私家侦探将会 能够使用她实验室开发的器官培养系统作为 所有这些目标的基本资源。首先，神经营养的作用 支持支配味觉的神经元发育的因素 芽对神经营养的依赖性与其他神经细胞不同 感觉神经节。这种依赖将通过形态测量来衡量 神经突起生长及临界生长发育的研究 感觉神经元的电生理特性。其次， 器官培养系统将允许对任何潜在的 胚胎舌上皮和间充质细胞的诱导作用 在味觉乳头的形成过程中。最后，两国之间的相互作用 随着味蕾的发育，感觉神经元的神经突起将不断生长 检查过了。后两个目标将通过建立混合 不同组织上皮、间充质或乳头、神经节的培养 年龄和形态发生潜能。这些数据将构成一个重要的主体 关于外周味觉系统发展的知识。

DESCRIPTION: (Adapted From the Applicants Abstract): This proposal deals with the role of several neurotrophic factors in the fetal development of sensory neurons that innervate taste buds, and interactions between fetal cell types in the morphogenesis of mature taste organs. The P.I. will be able to use an organ culture system developed in her laboratory as an essential resource for all these goals. Firstly, the role of neurotrophic factors in supporting the development of the neurons the innervate taste buds have a different neurotrophic dependence than neurons from other sensory ganglia. Such dependence will be measured in terms of morphometric studies on neurite outgrowth and in terms of the development of critical electrophysiological properties in the sensory neurons. Secondly, the organ culture system will allow detailed analyses of any potential induction roles that embryonic tongue epithelium and mesenchyme may play in the formation of gustatory papillae. Finally, the interactions of the growing neurites of sensory neurons with developing taste buds will be examined. The later two aims will be approached by establishing mixed cultures of epithelium and mesenchyme or papillae and ganglia of different ages and morphogenetic potential. These data will form a significant body of knowledge about the development of the peripheral gustator system.