SALT DEPENDENCE OF PROCESSIVITY OF T45G RIBONUCLEASE

苏氨酸45对甘氨酸合成能力的盐依赖性 使用31 P NMR测定核糖核酸酶A的（T45 G）突变 T45 G作用于聚腺苷（PolyA）的持续合成能力的基于试验 [S.B. delCardayr R.T. Raines，Biochemistry 33，6031-6037（1994）]。 的 NMR分析允许直接测定合成能力 参数，PI，在各种盐条件下的T45 G。 从 随着盐浓度的变化，PI的变化，可以评估 库仑相互作用的重要性 T45 G上的带电荷碱性残基与带负电荷的磷酸盐 对合成能力的影响。

The salt dependence of processivity of the Threonine 45 to Glycine (T45G) mutation of Ribonuclease A will be determined using a 31P NMR based assay for processivity of T45G acting on Polyadenosine (PolyA) [S.B. delCardayr R.T. Raines, Biochemistry 33, 6031-6037 (1994)]. The NMR assay allows for the direct determination of the processivity parameter, PI, of T45G at a variety of salt conditions. From the changes in PI with the changes in salt concentration, one can assess the importance of the coulombic interactions between the positively charged basic residues on T45G with the negatively charged phosphates on PolyA on processivity in this system.