CORE--MORPHOLOGY AND IMAGE ANALYSIS FACILITY

密歇根糖尿病研究中心的新图像分析核心(IAC)和 培训中心(MDRTC)是一个最先进的设施，它曾是 1991年在密歇根大学成立。它配备了 对接的现代光学和电子显微镜设施 拥有强大的计算机图像分析系统。《荧光报》 IAC的成像组配备了用于 细胞内各种离子的可视化和定量化 活细胞。IAC的目的是a)提供MDRTC 用形态计量学分析和定量细胞和 分子和离子的亚细胞定位，使用技术 定量组织化学、原位杂交和 荧光光度离子分析，b)向MDRTC提供咨询 用于设计和实施研究项目的调查员使用 这些技术，c)发起、实施并传播给MDRTC 调查人员采用创新的图像分析技术来促进 关于糖尿病、代谢和内分泌疾病的研究，以及d) 向MDRTC调查人员演示并传授上述技术， 实习生和技术人员。IAC的主要目标将是 提供最先进的定量形态分析，使用 分子探头，质量和发展优先。这个 IAC有望增强和刺激糖尿病及相关疾病 提供科学的内分泌学和新陈代谢研究 协作、专家协助和特殊设施 对于中心调查人员和受训人员的研究是必不可少的，但 这超出了他们个人的能力。

The new Image Analysis Core (IAC) of the Michigan Diabetes Research and Training Center (MDRTC) is a state-of-the-art facility, which was established at the University of Michigan in 1991. It is equipped with modern light and electron microscopic facilities which are interfaced with a powerful, computerized image analysis system. The Fluorescent Imaging Unit of the IAC is equipped with instruments for the visualization and quantification of various intracellular ions in living cells. The purpose of the IAC is to a) provide MDRTC investigators with morphometric analyses and quantitative cellular and subcellular localization of molecules and ions, using the techniques of quantitative histochemistry, in situ hybridization and fluorophotometric ion analysis, b) provide consultation to MDRTC investigators for the design and conduct of research projects using these techniques, c) initiate, implement and disseminate to MDRTC investigators new and innovative image analysis techniques to foster research on diabetes, metabolic and endocrine diseases, and d) demonstrate and teach the above techniques to MDRTC investigators, trainees and technicians. The major objective of the IAC will be to provide state-of-the-art quantitative morphological analyses using molecular probes, with quality and development having priority. The IAC is expected to enhance and stimulate diabetes and related endocrinology and metabolism research by providing scientific collaboration, expert assistance and special facilities that are essential for the research of Center investigators and trainees but that are beyond their individual capabilities.