DEVELOPMENTAL BIOLOGY AND GENETICS OF THE MAIZE LEAF

这些拟议研究的总体目标是了解 器官是在植物区段内设计的，以及如何区域性 能力是随着发展时间的推移而展开的。我们的系统，玉米 枝条和叶器官的发育，现在受益于骨骼 涉及10个克隆基因的功能网络，其中至少有8个 是转录因子。这些基因中的一些对于 初始化段的特定域。其中一个是必要的 使同源结构域基因在叶片中处于关闭状态。我们假设每个 同源框基因(Knox)在片段的不同区域起作用，以延缓 一份能力的“成熟时间表”。我们的建议集中在轴心上 以及我们的基因是否参与了长时间的- Range信号通路。目标编号L。I类NOX蛋白是如何 在节段和树叶中起作用？我们的战略包括跨越我们的 诺克斯基因敲除在一起，形成最小的线条。这些线条将 对免疫定位研究有价值，评估诺克斯丢失- 功能表型和用于测试诺克斯蛋白，运输。米饭 将被用作对照基因组。目标2。我们将确定是否 Rs2直接与Rs1基因结合。我们将使用酵母单杂交筛选 和蛋白质-DNA狭缝印迹。我们将制备一种抗RS2的抗体 蛋白质，以测试该Myb转录因子是否与 体内的诺克斯DNA。目标三。我们将进一步测试成熟度 地区性命运习得的时程假设与躯体 沿p-d轴的继承。我们期待着诺克斯函数的敲除 表型以加速发育。目标4。我们将按顺序 NS基因，并合作制备针对NS蛋白的抗体。目标 第五，我们将使用马赛克映射来发现， 表皮是ad-ab极性转换的“主宰”，它会标记 并克隆负责基因rld1。我们打算使用rld1基因 以进一步剖析其启动和维持的机制 背腹。目标6。我们将确认一个基因的克隆 参与接收涉及射击的远程信号 发育阶段变化(GEF1)，并将标记、克隆和研究LAX Midri1，其优势等位基因Lxm1-O是一种长程加速器 拍摄发展。我们正在阐明一种分子遗传学基础 异时性，将进化论与 发育生物学。

The general objective of these proposed studies is to understand how an organ is designed within the plant segment, and how regional competencies unfold over developmental time. Our system, the maize shoot segment and leaf organ development, now benefits from a skeletal network of function involving 10 cloned genes, at least eight of which are transcription factors. Some of these genes are essential for initializing particular domains of the segment. One is necessary to keep homeodomain genes "off' in the leaf. We hypothesize that each homeobox gene (knox) acts in varying regions of the segment to retard a "maturation schedule" of competence. Our proposal focuses on the axes of the leaf, and on whether or not our genes are involved in a long- range signaling pathway. Aim number l. How do Class I KNOX proteins function in the segment and leaf? Our strategy involves crossing our knox gene knockouts together to make minimized lines. These lines will be valuable for immunolocalization studies, assessing loss-of-KNOX- function phenotypes and for testing KNOX protein, trafficking. Rice will be used as a control genome. Aim number 2. We will determine if RS2 directly binds the rs1 gene. We will use a yeast one-hybrid screen and protein-DNA slot-blots. We will prepare an antibody against RS2 protein in order to test whether this Myb transcription factor binds knox DNA in vivo. Aim number 3. We will test further the maturation schedule hypothesis for regional fate acquisition and somatic inheritance along the p-d axis. We expect Knox function knockout phenotypes to accelerate development. Aim number 4. We will sequence ns genes and prepare an antibody to an NS protein, in collaboration. Aim number 5. We will use mosaic mapping to discover which, if either, epidermis is the "master" in switching ad-ab polarities, and will tag and clone the responsible gene, rld1. We intend to use the rld1 gene to further dissect the mechanism of initiation and maintenance of dorsiventrality. Aim number 6. We will confirm a clone of a gene involved in reception of long-range signals involving shoot developmental phase change (gef1), and will tag, clone and study lax midrib1, whose dominant allele Lxm1-O is a long-range accelerator of shoot development. We are elucidating a molecular genetic basis for heterochrony, the prevailing theory that links evolution with developmental biology.