Core--Instrumental and analytical measurements

仪器核心将为本计划的所有项目和主要研究人员提供：a)共聚焦和常规光学显微镜以及用于定位和共定位实验的固定样本的图像分析，以及形态变化的量化。B)活细胞比率荧光显微镜用于动态测量细胞内离子浓度的膜电位变化。C)微量注射蛋白质和核酸，以调节细胞内的信息或信号系统。D)在生物化学研究中，通过电穿孔将蛋白质注入细胞内以影响细胞内信号系统的调节。E)作为质量控制的上皮单层的常规阻力和电位测量。F)蛋白质和核援助凝胶和免疫印迹的定量分析。核心中心的工作人员将进行测量，并在规划测量和数据分析方面提供协助和咨询。

The Instrumentation Core will provide all projects and principle investigators of this Program Project Grant with: a) confocal and conventional light microscopy and image analyses of fixed samples for localization and colocalization experiments, as well as quantification of morphological changes. b) ratiometric fluorescence microscopy of live cells for dynamic measurements of intracellular ion concentration of membrane potential changes. c) microinjection of proteins and nucleic acids to modulate intracellular message or signaling systems. d) injection of proteins into cells by electroporation to effect modulation of intracellular signaling system in biochemical studies. e) routine resistance and potential measurements of epithelial monolayers as quality control. f) quantitative analyses of protein and nucleic aid gels and immuno-blots. The staff of the Core will carry out measurements and provide assistance and counseling in planning of measurements and data analyses.