BINDING ENERGY USE IN MECHANISM OF CATALYTIC ANTIBODY

我们正在研究我们实验室产生的一种催化抗体是如何利用 结合能促进催化作用。我们正在研究远程反应 居中互动。这些互动很有趣，因为大多数 半抗原设计已经被用来操纵活性部位 靠近反应中心。目前还没有关于 远程类型的绑定交互如何影响 催化机理或催化效率。我们使用MidasPlus 在计算机图形实验室中可视化的模型 我们实验室和其他实验室产生的催化抗体(AS 以及天然酶)，以帮助指导现场指导 突变和其他蛋白质工程研究。能够使用 这些结构也有助于我们理解 抗体催化和蛋白质催化的结构基础 总体而言是催化作用。

We are studying how a catalytic antibody generated in our lab uses binding energy to promote catalysis. We are studying remote reactive center interactions. These interactions are interesting because most hapten design has been geared toward manipulating the active site close to the reactive center. There have been no investigations about how the remote type of binding interactions can influence the catalytic mechanism or the efficiency of catalysis. We use MidasPlus in the Computer Graphics Laboratory to visualize models of the catalytic antibodies generated in our laboratory and in other labs (as well as natural enzymes) to assist in guiding site directed mutagenesis and other protein engineering studies. Being able to use these structures is also instrumental in helping us understand the structural basis of antibody catalysis as well as protein based catalysis in general.