EXTENSIONS OF MICROMACHINED MICROELECTRODE TECHNOLOGY

该项目旨在扩展基本的无源探头 通过给探测器提供更多的方法，使探测器变得更加通用的技术 与周围的细胞相互作用。我们已经开发出了 成功实现了一个允许包含 在与导电材料相同的衬底上的化学输送通道 电极位置。这允许多点药物输送和 用最少的组织进行多通道记录和/或刺激 颠覆。成功的化学品输送包装技术 已经开发出允许在正常情况下操作和使用的探头 神经细胞急性药理学特征的研究背景 活动。体外试验已经证实了量化的通过 向周围的介质注入剂量的液体。同时进行的体内试验 豚鼠下丘脑的化学递送和细胞外记录 和上丘显示的结果与 传统的方法。在会议上提交的数据，包括 1998年的耳鼻喉科研究协会已经产生了许多 神经科学界对该设备的兴趣。新设备 已伪造，其配置与特定的 将在Bledsoe博士的 实验室。过去一年的发展包括 一种在线流量计，用于监测输送的剂量化学物质。

This project is designed to extend the basic passive probe technology to make the probes more versatile by giving them more ways to interact with the cells which surround them. We have developed and successfully implemented a process which allows the inclusion of chemical delivery channels on the same substrate as the conductive electrode sites. This permits multipoint drug delivery and multichannel recording and/or stimulation with minimal tissue disruption. Successful packaging techniques for the chemical delivery probes have been developed which permit handling and use in the normal context of acute pharmacological characterization of neuronal activity. In vitro tests have verified the passage of quantified doses of fluid to a surrounding medium. In vivo tests of simultaneous chemical delivery and extracellular recordings in guinea pig inferior and superior colliculus are demonstrating results consistent with conventional approaches. Data presented at meetings including that of the 1998 Association for Research in Otolaryngology has generated much interest in the device by the neuroscience community. New devices have been fabricated which have configurations relevant to specific neurophysiological studies to be carried out in Dr. Bledsoe's laboratory. Developments during the past year include the development of an in-line flowmeter for monitoring of the dose chemical delivered.